中国环境科学
中國環境科學
중국배경과학
CHINA ENVIRONMENTAL SCIENCE
2014年
12期
3164-3174
,共11页
吴等等%宋志文%王琳%徐爱玲%夏岩
吳等等%宋誌文%王琳%徐愛玲%夏巖
오등등%송지문%왕림%서애령%하암
人工湿地%空气细菌%空气真菌%群落结构%基因文库
人工濕地%空氣細菌%空氣真菌%群落結構%基因文庫
인공습지%공기세균%공기진균%군락결구%기인문고
constructed wetland%airborne bacteria%airborne fungi%community structure%genomic library
通过构建16S/18S rDNA基因文库，分析自由表面流人工湿地污水处理系统春季空气细菌和空气真菌群落结构特征.结果表明，空气细菌分布在变形菌门(Proteobacteria)、放线菌门(Actinobacteria)、浮霉菌门(Planctomycetes)、蓝藻门(Cyanophyta)、绿弯菌门(Chloroflexi)、拟杆菌门(Bacteroidetes)和厚壁菌门(Firmicutes)，主要为β-变形菌纲(71.04%)、γ-变形菌纲(12.03%)、α-变形菌纲(3.83%)、蓝藻纲(4.38%)、芽孢杆菌纲(3.28%)和鞘脂杆菌纲(2.19%)，优势菌属是马赛菌属(Massilia 66.66%)、假单胞菌属(Pseudomonas 4.37%)、蓝丝细菌属(Cyanothece 3.83%)和沙雷氏菌属(Serratia 3.28%).空气真菌主要类群为座囊菌纲(Dothideomycetes 61.18%)，其次是接合菌纲(Zygomycetes 16.47%)、盘菌纲(Discomycetes 14.12%)，优势菌属是核腔菌属(Pyrenophora 48.31%)、被孢霉属(Mortierella 15.7%)、缘刺盘菌属(Cheilymenia 12.4%)、Boothiomyces (4.5%).人工湿地空气微生物中未检测出大肠杆菌(Escherichia coli)、沙门氏菌(Salmonella spp.)和产气荚膜梭菌(Clostridium perfringens)，但存在粘质沙雷氏菌(S. marcescens)、恶臭假单胞菌(P. putida)、表皮葡萄球菌(Staphylococcus epidermidis)等致病菌或条件致病菌.
通過構建16S/18S rDNA基因文庫，分析自由錶麵流人工濕地汙水處理繫統春季空氣細菌和空氣真菌群落結構特徵.結果錶明，空氣細菌分佈在變形菌門(Proteobacteria)、放線菌門(Actinobacteria)、浮黴菌門(Planctomycetes)、藍藻門(Cyanophyta)、綠彎菌門(Chloroflexi)、擬桿菌門(Bacteroidetes)和厚壁菌門(Firmicutes)，主要為β-變形菌綱(71.04%)、γ-變形菌綱(12.03%)、α-變形菌綱(3.83%)、藍藻綱(4.38%)、芽孢桿菌綱(3.28%)和鞘脂桿菌綱(2.19%)，優勢菌屬是馬賽菌屬(Massilia 66.66%)、假單胞菌屬(Pseudomonas 4.37%)、藍絲細菌屬(Cyanothece 3.83%)和沙雷氏菌屬(Serratia 3.28%).空氣真菌主要類群為座囊菌綱(Dothideomycetes 61.18%)，其次是接閤菌綱(Zygomycetes 16.47%)、盤菌綱(Discomycetes 14.12%)，優勢菌屬是覈腔菌屬(Pyrenophora 48.31%)、被孢黴屬(Mortierella 15.7%)、緣刺盤菌屬(Cheilymenia 12.4%)、Boothiomyces (4.5%).人工濕地空氣微生物中未檢測齣大腸桿菌(Escherichia coli)、沙門氏菌(Salmonella spp.)和產氣莢膜梭菌(Clostridium perfringens)，但存在粘質沙雷氏菌(S. marcescens)、噁臭假單胞菌(P. putida)、錶皮葡萄毬菌(Staphylococcus epidermidis)等緻病菌或條件緻病菌.
통과구건16S/18S rDNA기인문고，분석자유표면류인공습지오수처리계통춘계공기세균화공기진균군락결구특정.결과표명，공기세균분포재변형균문(Proteobacteria)、방선균문(Actinobacteria)、부매균문(Planctomycetes)、람조문(Cyanophyta)、록만균문(Chloroflexi)、의간균문(Bacteroidetes)화후벽균문(Firmicutes)，주요위β-변형균강(71.04%)、γ-변형균강(12.03%)、α-변형균강(3.83%)、람조강(4.38%)、아포간균강(3.28%)화초지간균강(2.19%)，우세균속시마새균속(Massilia 66.66%)、가단포균속(Pseudomonas 4.37%)、람사세균속(Cyanothece 3.83%)화사뢰씨균속(Serratia 3.28%).공기진균주요류군위좌낭균강(Dothideomycetes 61.18%)，기차시접합균강(Zygomycetes 16.47%)、반균강(Discomycetes 14.12%)，우세균속시핵강균속(Pyrenophora 48.31%)、피포매속(Mortierella 15.7%)、연자반균속(Cheilymenia 12.4%)、Boothiomyces (4.5%).인공습지공기미생물중미검측출대장간균(Escherichia coli)、사문씨균(Salmonella spp.)화산기협막사균(Clostridium perfringens)，단존재점질사뢰씨균(S. marcescens)、악취가단포균(P. putida)、표피포도구균(Staphylococcus epidermidis)등치병균혹조건치병균.
Airborne microbes are major biological components of the ecosystem and have important ecological functions. They affect human health, cause air pollution, and are key indicators of air quality. To determine the community structure of airborne microbes in a surface-flow wetland system constructed for sewage treatment in spring, the airborne bacteria and airborne fungi were analyzed by a 16S/18S rDNA gene clone library method. Phylogenetic analysis of airborne bacteria in the airborne microbes sample showed that there were seven groups: Proteobacteria, Actinobacteria, Planctomycetes, Cyanophyta, Chloroflexi, Bacteroidetes and Firmicutes. The main types of airborne bacteria were Betaproteobacteria (71.04%), Gammaproteobacteria (12.03%), Alphaproteobacteria (3.83%), Cyanophyceae (4.38%), Bacilli (3.28%) and Sphingobacteriales (2.19%), while the dominant genera of airborne bacteria included Massilia (66.66%), Pseudomonas (4.37%), Cyanothece (3.83%) and Serratia (3.28%). Phylogenetic analysis of airborne fungi in the airborne microbes sample showed that the main types were Dothideomycetes (61.18%), Zygomycetes (16.47%) and Discomycetes (14.12%). The dominant genera of airborne fungi included Pyrenophora (48.31%), Mortierella (15.7%), Cheilymenia (12.4%) and Boothiomyces (4.5%). Escherichia coli, Salmonella spp. and Clostridium perfringens were not detected in the sample of airborne microbe while other pathogens or conditioned pathogens, such as S. marcescens, p. putida, and Staphylococcus epidermidis were detected.
