中国计划生育和妇产科
中國計劃生育和婦產科
중국계화생육화부산과
CHINESE JOURNAL OF FAMILY PLANNING & GYNECOTOKOLOGY
2014年
9期
50-53
,共4页
REDD1%胎盘%mRNA,蛋白
REDD1%胎盤%mRNA,蛋白
REDD1%태반%mRNA,단백
REDD1%placenta%mRNA%protein
目的:探讨发育及DNA损伤反应调节基因1( regulated in development and DNA damage responses 1, REDD1)在人胎盘组织中的表达及其意义。方法选择早孕绒毛组织(6~10周)、中孕(24~28周)及足月晚孕(38~39周)胎盘组织各8例,采用免疫组织化学染色技术,Western blot杂交以及实时荧光定量聚合酶链反应( polymerase chain reaction, PCR)检测REDD1 mRNA及蛋白在不同孕周胎盘组织中的表达。结果 REDD1蛋白表达于胎盘滋养细胞的细胞质中,随着孕周增加,REDD1在mRNA及蛋白在胎盘组织中的表达呈现下降趋势。结论 REDD1可能早期参与了滋养细胞侵袭的过程。
目的:探討髮育及DNA損傷反應調節基因1( regulated in development and DNA damage responses 1, REDD1)在人胎盤組織中的錶達及其意義。方法選擇早孕絨毛組織(6~10週)、中孕(24~28週)及足月晚孕(38~39週)胎盤組織各8例,採用免疫組織化學染色技術,Western blot雜交以及實時熒光定量聚閤酶鏈反應( polymerase chain reaction, PCR)檢測REDD1 mRNA及蛋白在不同孕週胎盤組織中的錶達。結果 REDD1蛋白錶達于胎盤滋養細胞的細胞質中,隨著孕週增加,REDD1在mRNA及蛋白在胎盤組織中的錶達呈現下降趨勢。結論 REDD1可能早期參與瞭滋養細胞侵襲的過程。
목적:탐토발육급DNA손상반응조절기인1( regulated in development and DNA damage responses 1, REDD1)재인태반조직중적표체급기의의。방법선택조잉융모조직(6~10주)、중잉(24~28주)급족월만잉(38~39주)태반조직각8례,채용면역조직화학염색기술,Western blot잡교이급실시형광정량취합매련반응( polymerase chain reaction, PCR)검측REDD1 mRNA급단백재불동잉주태반조직중적표체。결과 REDD1단백표체우태반자양세포적세포질중,수착잉주증가,REDD1재mRNA급단백재태반조직중적표체정현하강추세。결론 REDD1가능조기삼여료자양세포침습적과정。
Objective To explore the significance of the expression of regulated in development and DNA damage responses 1 ( REDD1) in human placentas.Methods The study involved placental samples from different gestational weeks:early villous tissues ( gestational age:6 ~10 weeks) , second trimester ( gestational age:24 ~28 weeks) and third trimester ( gestational age: 38 ~39 weeks) placentas, with 8 cases in each group.REDD1 protein in the placenta was examined and located by immunohistochemistry and Western blot.Additionally, REDD1 mRNA expression level was determined by relative quantitative real time fluorescence PCR. REDD1 protein and mRNA expression levels were compared between the three groups.Results REDD1 protein was predominantly observed in the cytoplasm of villous cytotrophoblasts and trophoblasts.Compared with early pregnancy, expression of REDD1 protein and mRNA level decreased in middle and late pregnancy.Conclusion REDD1 may be early evolved in procedure of trophoblasts invasion.