CAJ | 학술논문

玻璃化冷冻法是在急速降温过程中使溶液行成一种无冰晶的玻璃化状态，可以最大程度避免冰晶形成对细胞膜及细胞骨架结构的损伤。影响卵巢组织玻璃化冷冻保存效果的主要因素有卵巢组织块的大小、冷冻保护剂的种类、渗透平衡方法、冷冻载体等。卵巢组织结构较复杂，尚未形成统一的玻璃化冷冻程序。提出了目前存在的问题。
파리화냉동법시재급속강온과정중사용액행성일충무빙정적파리화상태，가이최대정도피면빙정형성대세포막급세포골가결구적손상。영향란소조직파리화냉동보존효과적주요인소유란소조직괴적대소、냉동보호제적충류、삼투평형방법、냉동재체등。란소조직결구교복잡，상미형성통일적파리화냉동정서。제출료목전존재적문제。
Vitrification provides a rapid cooling to induce glass-like solidification inside cells and protect cell membrane and cytoskeleton system free of injury by ice crystals.The main factors that can influence the effect of vitrification are the size of ovarian tissue, the kind of cryoprotectants, the method of permeation, carrier system and so on. Ovarian tissue structure is very complex, and there is no uniform standardized protocol of vitrification yet.This paper presents also the current problems of ovarian tissue vitrification.