CAJ | 학술논문

目的：探讨小鼠整体胚胎和新生鼠石蜡切片技术的改进。方法收集不同胚龄和日龄的小鼠整体胚胎及新生鼠14.5、15.5及16.5 d的胚胎，清洗后直接置于4％中性甲醛中固定24 h以上；胚龄17.5、18.5 d及日龄0、1、2 d的小鼠，先用注射器往胸腹部及脑部缓慢注入固定液，再整体固定于标本瓶中24 h以上。经全自动密闭式组织脱水机编程脱水，石蜡包埋技术制备小鼠整体胚胎和新生鼠切片。应用HE染色方法对切片进行染色检验，光学显微镜下观察小鼠整体胚胎和新生鼠的不同组织结构。结果使用全自动密闭式组织脱水机处理小鼠整体胚胎和新生鼠，经过特定的脱水程序处理，镜下观察不同胚龄及日龄的不同组织器官，结构完整，层次清晰，一致性好。结论本实验室利用全自动密闭式组织脱水机对小鼠胚胎和新生鼠进行处理，通过设置合理程序，能够同时处理不同胎龄和日龄的小鼠整体胚胎及新生鼠；脱水后制备的石蜡切片结果稳定，一致性好，为小鼠遗传学和发育学研究提供了良好的技术支持。
목적：탐토소서정체배태화신생서석사절편기술적개진。방법수집불동배령화일령적소서정체배태급신생서14.5、15.5급16.5 d적배태，청세후직접치우4％중성갑철중고정24 h이상；배령17.5、18.5 d급일령0、1、2 d적소서，선용주사기왕흉복부급뇌부완만주입고정액，재정체고정우표본병중24 h이상。경전자동밀폐식조직탈수궤편정탈수，석사포매기술제비소서정체배태화신생서절편。응용HE염색방법대절편진행염색검험，광학현미경하관찰소서정체배태화신생서적불동조직결구。결과사용전자동밀폐식조직탈수궤처리소서정체배태화신생서，경과특정적탈수정서처리，경하관찰불동배령급일령적불동조직기관，결구완정，층차청석，일치성호。결론본실험실이용전자동밀폐식조직탈수궤대소서배태화신생서진행처리，통과설치합리정서，능구동시처리불동태령화일령적소서정체배태급신생서；탈수후제비적석사절편결과은정，일치성호，위소서유전학화발육학연구제공료량호적기술지지。
Objective To improve the method of paraffin section preparation of whole mouse embryos and newborn mice.Method Specimens of mouse embryos and newborn mice were collected.Embryos aged 14.5, 15.5 and 16.5 days were directly placed in 4%neutral formaldehyde and fixed for more than 24 hours.For embryos aged 17.5 and 18.5 days, and 0-, 1-and 2-day old newborn mice, a small amount of formaldehyde was slowly injected into the chest, abdomen and brain at first, respectively, and then the whole specimens were fixed for more than 24 hours.Tissues were processed together using a fully-enclosed tissue processor. Paraffin-embedded slices were observed under microscope after HE staining.Results The quality of specimens following the improved method was better than before.The structure of different tissues from both mouse embryos and newborn mice can be easily determined.No fragmentation was found in any organ tissue under the light microscope.Conclusions We can handle whole body specimens of both mouse embryos and newborn mice simultaneously by adjusting the program of the fully-enclosed tissue processor.The staining results of the sections which were dehydrated using this improved method are clear and stable.This improved method may provide a useful approach in research of genetics and developmental biology.