CAJ | 학술논문

目的：胫骨内注射Walker256肿瘤细胞制备骨癌痛大鼠模型，观察其脾脏T淋巴细胞的功能变化。方法健康雌性SD大鼠41只分两批实验进行。第一批实验动物16只，完全随机分为PBS组和模型组，模型组以胫骨内注射Walker256肿瘤细胞建立骨癌痛模型，PBS组仅注射相同剂量的无菌PBS。动态观察两组大鼠造模前、造模后4、6、8、10、12、14、16、18、20 d十个时间点的机械缩腿阈、热辐射刺激潜伏期和自发性疼痛。第二批实验动物25只，完全随机分为空白对照组、PBS组和模型组，观察各组造模后20 d脾脏T淋巴细胞增殖功能，T淋巴细胞及其亚群含量。结果第一批实验大鼠：造模前各组大鼠患侧足跖机械缩腿阈、热辐射刺激潜伏期和自发性疼痛差异无显著；造模后，模型组患侧足跖缩腿阈和自发性疼痛于模后4 d开始明显低于PBS组，并在整个实验过程中均低于PBS组，而其热辐射刺激潜伏期仅造模后8 d、10 d和12 d与PBS组比较有差异，其他时间点虽低于PBS组，但差异无显著性。第二批实验大鼠：PBS组大鼠脾脏T淋巴细胞增殖能力，T淋巴细胞（CD3）及其亚群（CD4、CD8）含量与空白对照组比较均差异无显著性；模型组大鼠的上述各项指标均少于空白对照组和PBS组，且其T淋巴细胞增殖能力显著弱于空白对照组和PBS组，CD3含量明显少于空白对照组。结论骨癌痛大鼠模型可出现明显的机械痛觉异常和自发性疼痛，其热痛觉异常仅发生在骨癌痛的中期；骨癌痛模型大鼠脾脏T淋巴细胞含量及其亚群均有不同程度的减弱。
목적：경골내주사Walker256종류세포제비골암통대서모형，관찰기비장T림파세포적공능변화。방법건강자성SD대서41지분량비실험진행。제일비실험동물16지，완전수궤분위PBS조화모형조，모형조이경골내주사Walker256종류세포건립골암통모형，PBS조부주사상동제량적무균PBS。동태관찰량조대서조모전、조모후4、6、8、10、12、14、16、18、20 d십개시간점적궤계축퇴역、열복사자격잠복기화자발성동통。제이비실험동물25지，완전수궤분위공백대조조、PBS조화모형조，관찰각조조모후20 d비장T림파세포증식공능，T림파세포급기아군함량。결과제일비실험대서：조모전각조대서환측족척궤계축퇴역、열복사자격잠복기화자발성동통차이무현저；조모후，모형조환측족척축퇴역화자발성동통우모후4 d개시명현저우PBS조，병재정개실험과정중균저우PBS조，이기열복사자격잠복기부조모후8 d、10 d화12 d여PBS조비교유차이，기타시간점수저우PBS조，단차이무현저성。제이비실험대서：PBS조대서비장T림파세포증식능력，T림파세포（CD3）급기아군（CD4、CD8）함량여공백대조조비교균차이무현저성；모형조대서적상술각항지표균소우공백대조조화PBS조，차기T림파세포증식능력현저약우공백대조조화PBS조，CD3함량명현소우공백대조조。결론골암통대서모형가출현명현적궤계통각이상화자발성동통，기열통각이상부발생재골암통적중기；골암통모형대서비장T림파세포함량급기아군균유불동정도적감약。
Objective To observe the functional changes of T lymphocytes in the spleen of rats with bone cancer pain.Methods forty-one healthy female Sprague-Dawley rats were used in this study, and were divided into blank control, PBS and Walker-256 tumor groups.Bone cancer pain model was established by inoculation of Walker 256 cancer cells into the tibial cavity.The paw withdrawal threshold (PWT), paw withdrawal thermal latency (PWL), and spontaneous pain (SP) were all measured before modelling (as base) and at 4, 6, 8, 10, 12, 14, 16, 18, and 20 days after modelling. The function of T lymphocyte proliferation, and the content of T lymphocytes and their subgroups in the spleen were detected by cell counting kit-8 method and flow cytometry, respectively, on day 20 after modelling.Results Before modellng, there were no differences of PWT, PWL, and SP between the PBS and model groups.After modelling, the PWT and SP of model group were significantly decreased on day 4, and were always lower than that of PBS group during the experiment.Statistical analysis revealed that Walker-256 cancer cell inoculation in the tibia induced a significant decrease in PWL on day 8, 10 and 12 after modellng.Compared with the control group, T lymphocyte proliferation, content of T lymphocyte (CD3) and subgroups ( CD4 and CD8) in the PBS group were not significantly decreased.However, T lymphocyte proliferation and the content of CD3 lymphocytes in the model group were significantly lower than those in the blank control group and/or PBS group.Conclusions The bone cancer pain rat model may appear obvious mechanical allodynia and spontaneous pain.Its thermal pain hyperalgesiaonly occurred in the intermediate stage of bone cancer pain. The content of T lymphocytes and its subgroups, and the function of T lymphocyte proliferation are weakened to some extent in the bone cancer pain rat model.