CAJ | 학술논문

目的：筛选出适于监测无束缚蟾蜍动态心电的导联方式，并分析其实用性。方法：蟾蜍在麻醉状态下皮下包埋引导电极后，通过BL-420 S生物机能实验系统监测了5种导联的动态心电，比较并筛选出能够较好地显示心电波形的导联。利用该导联监测了蟾蜍从人工冬眠中复苏6h内的动态心电和连续5d内的心率和心率变异性（ heart rate variability， HRV）的日间稳定性，以及冻融处理前后的心率和HRV，以分析其实用性。结果：5种备选导联中有2种导联能较好显示心电波形。与人工冬眠复苏6 h比较，复苏1 h之内的心率降低，窦性心率RR间期标准差（ the standard deviation of normal R-R intervals， SDNN）升高，二者均有显著差异（ P＜0.05或P＜0.01）；2 h到5 h之间的心率和SDNN无显著变化，提示心电在复苏2 h后恢复稳定。冬眠第4天和第5天的2 h和4 h心率较第1天显著减慢（P＜0.05或P＜0.01），而SDNN较第1天显著升高（P＜0.05或P＜0.01），提示留置电极的蟾蜍心电在3 d内不会有显著变化。与冷冻前比较，解冻1 h和12 h的心率显著加快（P＜0.05），SDNN显著变小（P＜0.05），表明冻融后蟾蜍心功能下降。结论：皮下植入电极的方法可有效监测蟾蜍动态心电。
목적：사선출괄우감측무속박섬서동태심전적도련방식，병분석기실용성。방법：섬서재마취상태하피하포매인도전겁후，통과BL-420 S생물궤능실험계통감측료5충도련적동태심전，비교병사선출능구교호지현시심전파형적도련。이용해도련감측료섬서종인공동면중복소6h내적동태심전화련속5d내적심솔화심솔변이성（ heart rate variability， HRV）적일간은정성，이급동융처리전후적심솔화HRV，이분석기실용성。결과：5충비선도련중유2충도련능교호현시심전파형。여인공동면복소6 h비교，복소1 h지내적심솔강저，두성심솔RR간기표준차（ the standard deviation of normal R-R intervals， SDNN）승고，이자균유현저차이（ P＜0.05혹P＜0.01）；2 h도5 h지간적심솔화SDNN무현저변화，제시심전재복소2 h후회복은정。동면제4천화제5천적2 h화4 h심솔교제1천현저감만（P＜0.05혹P＜0.01），이SDNN교제1천현저승고（P＜0.05혹P＜0.01），제시류치전겁적섬서심전재3 d내불회유현저변화。여냉동전비교，해동1 h화12 h적심솔현저가쾌（P＜0.05），SDNN현저변소（P＜0.05），표명동융후섬서심공능하강。결론：피하식입전겁적방법가유효감측섬서동태심전。
[ ABSTRACT] AIM:To screen out a suitable lead for monitoring the ambulatory electrocardiogram ( ECG) in un-restrained toad, and to investigate its practicability.METHODS:After subcutaneously implanting the electrodes in toads under anaesthesia, the ambulatory ECG of 5 leads were monitored with BL-420S data acquisition and analysis system, and the leads which could well express the waveform in ECG were screened out.The recovery process of the toads from the arti-ficial hibernation within 6 h, the day-to-day stability of the heart rate ( HR) and the heart rate variability ( HRV) in 5 suc-cessive days of hibernation, and the HR and HRV after freeze-thawing process were monitored to determine its practicabili-ty.RESULTS:Two out of 5 leads showed better ECG waveforms.Compared with 6 h post hibernation, lowered HR at 0 h and 1 h was observed, and the standard deviation of normal R-R intervals ( SDNN) was significantly increased ( P<0.05 or P<0.01), but the HR and SDNN from 2 to 5 h showed no significant difference, suggesting that the cardiac function reached the steady state after 2 h recovery.The HR at 2 h and 4 h on day 4 and day 5 decreased significantly compared with that on day 1 (P<0.05 or P<0.01), followed with a significant increase in SDNN (P<0.05 or P<0.01), sugges-ting that the ECG remained stable within 3 d.The HR increased, while SDNN decreased significantly at 1 h and 12 h post-thawing compared with that at pre-freeze (P<0.05), indicating the damaged cardiac function after freeze-thawing process. CONCLUSION:The method of subcutaneously implanting electrodes is suitable for effectively monitoring the ambulatory ECG in toads.