CAJ | 학술논문

目的：建立胰岛素抵抗大鼠模型，探讨血清网膜素-1水平与胰岛素抵抗的相关性。方法：30只SPF级雄性Wistar大鼠随机分为正常对照组（NC，n＝15）、高脂饮食组（HF，n＝15），自由摄食、饮水，NC组给予普通饲料，HF组给予高脂饲料诱导胰岛素抵抗模型。10周后2组各取5只行高胰岛素正葡萄糖钳夹实验评估模型， ELISA法检测血清网膜素-1，放射免疫法检测血清基础胰岛素。结果：（1）各组大鼠基础血糖比较无显著差异（P＞0.05）。（2）HF组血清基础胰岛素水平较NC组明显升高（P＜0.05），但血清网膜素-1水平较NC组明显降低（P＜0.01）。（3）Pearson相关性分析示血清网膜素-1分别与胰岛素（r＝－0.654，P＜0.01）和游离脂肪酸（r＝－0.446，P＜0.05）呈负相关。结论：在大鼠胰岛素抵抗阶段，血清网膜素-1水平已经开始降低；基础胰岛素水平随着血清网膜素-1水平的降低而升高，推测血清网膜素-1水平降低可能成为胰岛素抵抗、糖尿病及心血管疾病的早期提示因子。
목적：건립이도소저항대서모형，탐토혈청망막소-1수평여이도소저항적상관성。방법：30지SPF급웅성Wistar대서수궤분위정상대조조（NC，n＝15）、고지음식조（HF，n＝15），자유섭식、음수，NC조급여보통사료，HF조급여고지사료유도이도소저항모형。10주후2조각취5지행고이도소정포도당겸협실험평고모형， ELISA법검측혈청망막소-1，방사면역법검측혈청기출이도소。결과：（1）각조대서기출혈당비교무현저차이（P＞0.05）。（2）HF조혈청기출이도소수평교NC조명현승고（P＜0.05），단혈청망막소-1수평교NC조명현강저（P＜0.01）。（3）Pearson상관성분석시혈청망막소-1분별여이도소（r＝－0.654，P＜0.01）화유리지방산（r＝－0.446，P＜0.05）정부상관。결론：재대서이도소저항계단，혈청망막소-1수평이경개시강저；기출이도소수평수착혈청망막소-1수평적강저이승고，추측혈청망막소-1수평강저가능성위이도소저항、당뇨병급심혈관질병적조기제시인자。
[ ABSTRACT] AIM:To establish the insulin resistance rat model for evaluating the correlation of omentin-1 level and insulin resistance.METHODS: SPF male Wistar rats ( n =30 ) were randomly divided into normal control group (NC, n=15) and high-fat diet group (HF, n=15).The rats in NC group were fed with basic diet.The insulin resistant model was established by feeding the rats with high-fat diet in HF group.After 10 weeks, 5 rats in each group were as-sessed by the technique of hyperinsulinaemic-euglycaemic clamp.After the insulin resistant model was successfully estab-lished, the body weight and fasting blood glucose were detected.The concentration of fasting serum omentin-1 was analyzed by ELISA.Fasting serum insulin was measured by radioimmunoassay.RESULTS: No difference of fasting blood glucose between the 2 groups was observed.The level of fasting serum insulin in HF group was significantly higher than that in NC group ( P <0.05 ) .The level of serum omentin-1 in HF group were significantly decreased compared with NC group (P<0.01).Pearson’s correlation analysis showed that negative correlations between serum omentin-1 and fasting serum insulin (r=-0.654,P<0.01), serum omentin-1 and free fatty acid (r=-0.446, P<0.05) was found.CONCLU-SION:In rats, serum omentin-1 level began to decrease at insulin resistance stage.As serum omentin-1 level decreased, the basal insulin level increased, indicating that decreased serum omentin-1 level may be an early factor of IR, diabetes and cardiovascular diseases.