CAJ | 학술논문

目的：探讨富含亮氨酸重复序列的G蛋白偶联受体5（ Lgr5）在慢性浅表性胃炎中的表达及其临床意义。方法：将30只Wistar大鼠随机分为空白组、模型组和对照组。采用0.02％氨水合并饥饱失常法对大鼠进行慢性浅表性胃炎造模，模型复制成功后，采用免疫组化、Western blotting和RT-PCR检测各组中Lgr5的mRNA及蛋白的表达水平。结果：Lgr5主要在细胞的胞膜和胞浆中表达，模型组中Lgr5 mRNA和蛋白表达水平高于对照组和空白组大鼠（P＜0.01），对照组和空白组中Lgr5表达水平差异不显著（P＞0.05）。结论：持续的炎症刺激导致Lgr5表达增加，Lgr5可能是一种炎性的促肿瘤因子。
목적：탐토부함량안산중복서렬적G단백우련수체5（ Lgr5）재만성천표성위염중적표체급기림상의의。방법：장30지Wistar대서수궤분위공백조、모형조화대조조。채용0.02％안수합병기포실상법대대서진행만성천표성위염조모，모형복제성공후，채용면역조화、Western blotting화RT-PCR검측각조중Lgr5적mRNA급단백적표체수평。결과：Lgr5주요재세포적포막화포장중표체，모형조중Lgr5 mRNA화단백표체수평고우대조조화공백조대서（P＜0.01），대조조화공백조중Lgr5표체수평차이불현저（P＞0.05）。결론：지속적염증자격도치Lgr5표체증가，Lgr5가능시일충염성적촉종류인자。
[ ABSTRACT] AIM: To investigate the expression and significance of Leucine-rich repeat-containing G-protein-coupled receptor 5 (Lgr5) in chronic superficial gastritis.METHODS:The Wistar rats (n=30) were randomly divided into blank group, model group and control group.The Wistar rat model of chronic superficial gastritis was established by in-tragastric administration of 0.02%ammonia and long-term irregular diet.All rats were sacrificed, and gastric tissues were harvested and stained with hematoxylin and eosin.The expression of Lgr5 at mRNA and protein levels was analyzed by re-verse transcriptional polymerase chain reaction, Western blotting and immunohistochemistry.RESULTS:Lgr5 was mainly expressed in the cell membrane and cytoplasm.Lgr5 showed high expression in model group compared with blank group and control group.No obvious difference between blank group and control group was observed.CONCLUSION:Persistent in-flammation leads to increased expression of Lgr5.Lgr5 may be a proinflammatory tumor promoting factor.