CAJ | 학술논문

目的：研究白细胞分化抗原（CD）147在体外对前列腺癌PC-3细胞的自噬作用。方法：通过氨基酸饥饿法建立自噬模型，免疫印迹技术检测CD147的表达。利用RNA干扰CD147表达的细胞系，免疫印迹技术检测自噬蛋白LC3-I和LC3-II的表达；台盼蓝排斥实验检测细胞的死亡情况。结果：在PC-3细胞自噬模型中，随着饥饿诱导时间延长，CD147表达逐渐升高。用RNA干扰技术降低CD147表达后，与阴性对照组比较，在自噬模型中CD147干扰组自噬相关蛋白LC3-II表达增多；并且细胞死亡数量明显增加，阴性对照组细胞死亡率分别为（19.3±3.1）％和（22.3±3.5）％，而在CD147干涉组细胞死亡率为（38.4±3.1）％，差异有统计学意义（P＜0.05）。结论：在前列腺癌PC-3细胞中CD147抑制饥饿诱导的自噬，减少自噬性细胞死亡的发生。
목적：연구백세포분화항원（CD）147재체외대전렬선암PC-3세포적자서작용。방법：통과안기산기아법건립자서모형，면역인적기술검측CD147적표체。이용RNA간우CD147표체적세포계，면역인적기술검측자서단백LC3-I화LC3-II적표체；태반람배척실험검측세포적사망정황。결과：재PC-3세포자서모형중，수착기아유도시간연장，CD147표체축점승고。용RNA간우기술강저CD147표체후，여음성대조조비교，재자서모형중CD147간우조자서상관단백LC3-II표체증다；병차세포사망수량명현증가，음성대조조세포사망솔분별위（19.3±3.1）％화（22.3±3.5）％，이재CD147간섭조세포사망솔위（38.4±3.1）％，차이유통계학의의（P＜0.05）。결론：재전렬선암PC-3세포중CD147억제기아유도적자서，감소자서성세포사망적발생。
[ ABSTRACT] AIM: To study the autophagy of prostate cancer PC-3 cells induced by CD147 in vitro.ME-THODS:Themethod of amino acid starvation to induce autophagy was used.The expression of CD147 was detected by Western blotting.To study the functional effects of CD147 on autophagy in prostate cancer PC-3 cells, the down-regulation of CD147 expression was induced by the technique of RNAi.The conversion of autophagic marker protein LC3-I to LC3-II was determined by Western blotting.The cell death after starvation-induced autophagy was analyzed by trypan blue exclu-sion assay.RESULTS:The CD147 expression gradually increased in starvation-induced autophagy.The down-regulation of CD147 significantly increased the expression of autophagy-related protein LC3-II compared with control group.Mean-while, the cell death rates increased from (19.3 ±3.1)%and (22.3 ±3.5)%in control groups to (38.4 ±3.1)%in si-lencing the expression of CD147 in the PC-3 cells (P<0.05).CONCLUSION:CD147 inhibits starvation-induced auto-phgy and autophagy death in the prostate cancer PC-3 cells.