中国病理生理杂志
中國病理生理雜誌
중국병리생리잡지
CHINESE JOURNAL OF PATHOPHYSIOLOGY
2014年
12期
2155-2160
,共6页
刘丽璇%吴灵飞%邓巍%周小涛%陈锐沛%项梦琦%郭益添%蒲泽锦%李国平
劉麗璇%吳靈飛%鄧巍%週小濤%陳銳沛%項夢琦%郭益添%蒲澤錦%李國平
류려선%오령비%산외%주소도%진예패%항몽기%곽익첨%포택금%리국평
丹参酮IIA%低氧%HepG2细胞%细胞凋亡
丹參酮IIA%低氧%HepG2細胞%細胞凋亡
단삼동IIA%저양%HepG2세포%세포조망
Tanshinone IIA%Hypoxia%HepG2 cells%Apoptosis
目的：探讨低氧条件下丹参酮IIA（ Tan IIA）对人肝癌HepG2细胞增殖和凋亡的影响及其分子机制。方法：用氯化钴（ CoCl2）创建低氧模型，实验分为常氧对照组、低氧对照组和低氧＋Tan IIA处理组。不同浓度的Tan IIA分别作用于低氧下人肝癌HepG2细胞24 h、48 h和72 h，采用MTT法测定Tan IIA对低氧下HepG2细胞增殖的抑制作用。不同浓度的Tan IIA分别作用于低氧条件下HepG2细胞24 h和48 h后，Hoechst 33258染色法检测细胞核的形态学变化并计算凋亡率。不同浓度的Tan IIA作用于低氧条件下人肝癌HepG2细胞48 h后，Western blotting检测低氧诱导因子1α（ HIF-1α）、血管内皮生长因子（ VEGF）和野生型P53的蛋白表达情况。结果：低氧条件下Tan IIA以时间和剂量依赖方式抑制HepG2细胞的生长和增殖。 Tan IIA作用于低氧下的HepG2细胞后，可见典型的凋亡细胞形态学特征，细胞凋亡率呈时间、剂量依赖性的增加。 Western blotting免疫印迹法显示常氧对照组的HIF-1α和VEGF表达较低，而低氧对照组的HIF-1α、VEGF蛋白表达较常氧组升高，低氧下随着Tan IIA 浓度的升高，HIF-1α和VEGF蛋白的表达明显降低，野生型P53蛋白的表达随着Tan IIA浓度的升高而升高。结论：低氧条件下，Tan IIA能抑制肝癌HepG2细胞增殖并诱导其凋亡，其机制可能与抑制HIF-1α和VEGF蛋白表达，上调P53蛋白表达有关。
目的：探討低氧條件下丹參酮IIA（ Tan IIA）對人肝癌HepG2細胞增殖和凋亡的影響及其分子機製。方法：用氯化鈷（ CoCl2）創建低氧模型，實驗分為常氧對照組、低氧對照組和低氧＋Tan IIA處理組。不同濃度的Tan IIA分彆作用于低氧下人肝癌HepG2細胞24 h、48 h和72 h，採用MTT法測定Tan IIA對低氧下HepG2細胞增殖的抑製作用。不同濃度的Tan IIA分彆作用于低氧條件下HepG2細胞24 h和48 h後，Hoechst 33258染色法檢測細胞覈的形態學變化併計算凋亡率。不同濃度的Tan IIA作用于低氧條件下人肝癌HepG2細胞48 h後，Western blotting檢測低氧誘導因子1α（ HIF-1α）、血管內皮生長因子（ VEGF）和野生型P53的蛋白錶達情況。結果：低氧條件下Tan IIA以時間和劑量依賴方式抑製HepG2細胞的生長和增殖。 Tan IIA作用于低氧下的HepG2細胞後，可見典型的凋亡細胞形態學特徵，細胞凋亡率呈時間、劑量依賴性的增加。 Western blotting免疫印跡法顯示常氧對照組的HIF-1α和VEGF錶達較低，而低氧對照組的HIF-1α、VEGF蛋白錶達較常氧組升高，低氧下隨著Tan IIA 濃度的升高，HIF-1α和VEGF蛋白的錶達明顯降低，野生型P53蛋白的錶達隨著Tan IIA濃度的升高而升高。結論：低氧條件下，Tan IIA能抑製肝癌HepG2細胞增殖併誘導其凋亡，其機製可能與抑製HIF-1α和VEGF蛋白錶達，上調P53蛋白錶達有關。
목적：탐토저양조건하단삼동IIA（ Tan IIA）대인간암HepG2세포증식화조망적영향급기분자궤제。방법：용록화고（ CoCl2）창건저양모형，실험분위상양대조조、저양대조조화저양＋Tan IIA처리조。불동농도적Tan IIA분별작용우저양하인간암HepG2세포24 h、48 h화72 h，채용MTT법측정Tan IIA대저양하HepG2세포증식적억제작용。불동농도적Tan IIA분별작용우저양조건하HepG2세포24 h화48 h후，Hoechst 33258염색법검측세포핵적형태학변화병계산조망솔。불동농도적Tan IIA작용우저양조건하인간암HepG2세포48 h후，Western blotting검측저양유도인자1α（ HIF-1α）、혈관내피생장인자（ VEGF）화야생형P53적단백표체정황。결과：저양조건하Tan IIA이시간화제량의뢰방식억제HepG2세포적생장화증식。 Tan IIA작용우저양하적HepG2세포후，가견전형적조망세포형태학특정，세포조망솔정시간、제량의뢰성적증가。 Western blotting면역인적법현시상양대조조적HIF-1α화VEGF표체교저，이저양대조조적HIF-1α、VEGF단백표체교상양조승고，저양하수착Tan IIA 농도적승고，HIF-1α화VEGF단백적표체명현강저，야생형P53단백적표체수착Tan IIA농도적승고이승고。결론：저양조건하，Tan IIA능억제간암HepG2세포증식병유도기조망，기궤제가능여억제HIF-1α화VEGF단백표체，상조P53단백표체유관。
[ ABSTRACT] AIM:To investigate the effects of tanshinone IIA ( Tan IIA) on proliferation, apoptosis and its mo-lecular mechanism in human hepatoma HepG2 cells under hypoxic condition.METHODS:Hypoxia model was established by treatment with cobalt chloride ( CoCl2 ) .The cells were divided into normoxia control group, hypoxia control group and hypoxia combined at different concentrations of Tan IIA groups.After HepG2 cells were incubated with different concentra-tions of Tan IIA (0.5, 1.0, 2.0, 5.0 and 10.0 mg/L) for 24 h, 48 h and 72 h under hypoxic condition, the cell prolifer-ation was determined by MTT assay.After Tan IIA was added to the media at different concentrations for 24 h and 48 h, the apoptotic cells were observed by Hoechst 33258 staining.The protein levels of hypoxia-inducible factor 1 alpha (HIF-1α) , vascular endothelial growth factor ( VEGF) and wild-type P53 were detected by Western blotting after cultured with different concentrations of Tan IIA for 48 h.RESULTS:Tan IIA inhibited the proliferation of HepG2 cells in a dose-and time-dependent manner.Tan IIA induced the typical morphology of apoptotic cells and increased the apoptotic rate in a dose-and time-dependent manner after treatment with 1.0 mg/L~5.0 mg/L for 24 h and 48 h under hypoxic condition. The protein levels of HIF-1αand VEGF were weakly expressed in HepG2 cells under normoxia but up-regulated after incu-bated under hypoxia for 48 h.The protein expression of HIF-1αand VEGF were decreased with the increase in the concen-tration of Tan IIA under hypoxia.The protein expression of wild-type P53 was increased with the increase in the concentra-tions of Tan IIA under hypoxia.CONCLUSION:Tan IIA significantly inhibits the proliferation and induces the apoptosis of human hepatoma HepG2 cells under hypoxia, which may be related to the down-regulation of HIF-1αand VEGF and up-regulation of wild-type P53.
