CAJ | 학술논문

目的：研究胰岛素抗性在原发性肝癌细胞中的生物学功能及对顺铂抗癌药物的抗药敏感性的影响。方法：利用高浓度的胰岛素连续培养HepG2细胞72 h获得抗胰岛素的HepG2细胞（ HepG2／IR），检测HepG2／IR细胞的黏附、迁移和侵袭的能力改变以及对顺铂的敏感性；同时利用流式细胞术测定HepG2和HepG2／IR中胰岛素受体和葡萄糖转运蛋白2的表达情况。结果：胰岛素抗性HepG2／IR细胞中葡萄糖消耗量显著降低；胰岛素受体和葡萄糖转运蛋白2表达下调；而HepG2／IR细胞的黏附、迁移和侵袭能力明显增强；同时HepG2／IR细胞对顺铂的敏感性降低；但用吡格列酮处理HepG2／IR细胞后其黏附、迁移和侵袭能力显著减弱。结论：胰岛素抗性与HepG2细胞的耐药性、细胞黏附、迁移和侵袭能力密切相关。这有助于解释具有胰岛素抗性的癌症患者对化疗失敏的临床现象。
목적：연구이도소항성재원발성간암세포중적생물학공능급대순박항암약물적항약민감성적영향。방법：이용고농도적이도소련속배양HepG2세포72 h획득항이도소적HepG2세포（ HepG2／IR），검측HepG2／IR세포적점부、천이화침습적능력개변이급대순박적민감성；동시이용류식세포술측정HepG2화HepG2／IR중이도소수체화포도당전운단백2적표체정황。결과：이도소항성HepG2／IR세포중포도당소모량현저강저；이도소수체화포도당전운단백2표체하조；이HepG2／IR세포적점부、천이화침습능력명현증강；동시HepG2／IR세포대순박적민감성강저；단용필격렬동처리HepG2／IR세포후기점부、천이화침습능력현저감약。결론：이도소항성여HepG2세포적내약성、세포점부、천이화침습능력밀절상관。저유조우해석구유이도소항성적암증환자대화료실민적림상현상。
[ ABSTRACT] AIM:To investigate the effect of insulin resistance ( IR) on the biological function of hepatocellu-lar carcinoma ( HCC) and sensitivity to cisplatin.METHODS:IR was induced in HepG2 cells via incubation with a high concentration of insulin.Afterwards, the effects of IR on adhesion, migration, invasion and sensitivity to cisplatin of the cells were detected.RESULTS:The results indicated that glucose consumption was reduced in the IR cells.The expres-sion of the insulin receptor and glucose transporter 2 was down-regulated.Furthermore, HepG2/IR cells displayed marked-ly enhanced adhesion, migration, and invasion.These cells exhibited a lower sensitivity to cisplatin.On the contrary, HepG2/IR cells exhibited decreased adhesion and invasion after treatment with the insulin sensitizer pioglitazone hydrochlo-ride.CONCLUSION:IR is closely related to drug resistance, adhesion, migration and invasion in HepG2 cells.These findings may help explain the clinical observation of the limited efficacy of chemotherapy on a background of IR.