CAJ | 학술논문

目的：观察平原短期高强度间歇运动训练（ high-intensity interval training， HIT）对青年男性急进高原淋巴细胞线粒体自噬的影响。方法30名世居平原青年男性随机分为无运动训练组（ NT组，n＝15）和HIT组（ n＝15）。 HIT组受试者进行HIT训练，以90％～95％心率贮备强度快跑3 min，间歇以45％～50％心率贮备强度慢跑3 min，重复6个循环，1次／d，共2周。 NT组除日常活动外不进行任何形式运动训练。训练后所有受试者同批次急进高原，在移居高原24 h检测淋巴细胞线粒体复合体活性，以及PINK1、淋巴细胞线粒体自噬蛋白Bnip3和Beclin-1蛋白表达。结果与NT组比较，HIT组Bnip3（100±18 vs 136±32）和Beclin-1（100±19 vs 119±26）表达明显升高（P均＜0．05），线粒体复合体Ⅰ、Ⅱ、Ⅳ和ATP合成酶活性也明显升高（P＜0．05），PINK1蛋白表达明显降低（P＜0．01）。结论平原阶段短期HIT可通过上调线粒体自噬阈，从而减少高原低氧环境下淋巴细胞线粒体损伤，提高线粒体能量代谢水平。
목적：관찰평원단기고강도간헐운동훈련（ high-intensity interval training， HIT）대청년남성급진고원림파세포선립체자서적영향。방법30명세거평원청년남성수궤분위무운동훈련조（ NT조，n＝15）화HIT조（ n＝15）。 HIT조수시자진행HIT훈련，이90％～95％심솔저비강도쾌포3 min，간헐이45％～50％심솔저비강도만포3 min，중복6개순배，1차／d，공2주。 NT조제일상활동외불진행임하형식운동훈련。훈련후소유수시자동비차급진고원，재이거고원24 h검측림파세포선립체복합체활성，이급PINK1、림파세포선립체자서단백Bnip3화Beclin-1단백표체。결과여NT조비교，HIT조Bnip3（100±18 vs 136±32）화Beclin-1（100±19 vs 119±26）표체명현승고（P균＜0．05），선립체복합체Ⅰ、Ⅱ、Ⅳ화ATP합성매활성야명현승고（P＜0．05），PINK1단백표체명현강저（P＜0．01）。결론평원계단단기HIT가통과상조선립체자서역，종이감소고원저양배경하림파세포선립체손상，제고선립체능량대사수평。
Objective To explore the effect of short-term high-intensity interval training ( HIT) in plain on mitochondrial au-tophagy in leukocyte after rush entry into plateau.Methods Thirty male lowlanders were randomly divided into two groups:untrained group (NT, n=15) and HIT group (n=15).Participants in the HIT group run at a high training intensity, 90%-95%heart rate re-serve ( HRR) for 3 min, with active pauses of 3 min of walking at 45%-50% of HRR.Participants in the HIT group underwent six sessions of HIT for 36 min/d, 7 days per week for 2 weeks.After training, participants in both HIT and NT groups traveled into plat-eau in the same batch.Activities of mitochondrial complex, PINK1, Bnip3 and Beclin-1 protein expression in leukocyte were measured in plateau at 24 h after entry.Results As compared with NT group, in HIT group, mitochondrial autophagy proteins Bnip3 (100 ±18 vs 136 ±32) and Beclin-1(100 ±19 vs 119 ±26) expression were significantly elevated(P<0.05).Furthermore, mitochondrial complexⅠ,Ⅱ,Ⅳand ATP synthase activities were significantly elevated (P<0.05), whereas PINK1 protein expression was significantly decreased (P<0.01).Conclusions Short-term HIT in plain can blunt high altitude hypoxia induced mitochondrial impariment and elevated mitochondrial energy metabolism through upregulation of mitochondrial autophagy.