CAJ | 학술논문

目的：探讨宝石 CT 能谱成像定量测定小鼠脂肪肝模型肝脏脂肪含量的可行性。方法对24只不同程度脂肪肝小鼠模型行宝石 CT 能谱成像,测定肝实质混合能量下 CT 值,65 keV 下 CT 值,各种物质分析(脂/水,脂/碘,脂/钙基物质配对)中的脂肪含量,并获取能谱曲线。取小鼠肝脏标本进行甘油三酯(triglyceride,Tg)含量测定及苏木精-伊红染色法(hematoxylin-eosinstain-ing,HE),分析能谱 CT 各种指标与 Tg 含量之间的相关性。结果脂/水配对脂含量与 Tg 的相关性(r=0.915)优于65 keV 下 CT值(r=-0.858)及混合能量下 CT 值(r=-0.816),而脂/碘、脂/钙配对脂含量与 Tg 的相关性较低(r =-0.726及-0.660),以上统计学指标 P <0.001。1.25 mm 层厚 CT 图像各指标比2.5 mm 层厚相关性更高。随着脂肪肝程度的不断加重,能谱曲线的形态不断发生变化。结论宝石 CT 能谱成像能够无创、准确、定量检测脂肪肝小鼠肝脏脂肪含量。
목적：탐토보석 CT 능보성상정량측정소서지방간모형간장지방함량적가행성。방법대24지불동정도지방간소서모형행보석 CT 능보성상,측정간실질혼합능량하 CT 치,65 keV 하 CT 치,각충물질분석(지/수,지/전,지/개기물질배대)중적지방함량,병획취능보곡선。취소서간장표본진행감유삼지(triglyceride,Tg)함량측정급소목정-이홍염색법(hematoxylin-eosinstain-ing,HE),분석능보 CT 각충지표여 Tg 함량지간적상관성。결과지/수배대지함량여 Tg 적상관성(r=0.915)우우65 keV 하 CT치(r=-0.858)급혼합능량하 CT 치(r=-0.816),이지/전、지/개배대지함량여 Tg 적상관성교저(r =-0.726급-0.660),이상통계학지표 P <0.001。1.25 mm 층후 CT 도상각지표비2.5 mm 층후상관성경고。수착지방간정도적불단가중,능보곡선적형태불단발생변화。결론보석 CT 능보성상능구무창、준학、정량검측지방간소서간장지방함량。
Objective To investigate the feasibility of quantitative measurement of fat concentration by CT spectral imaging in a mice model of fatty liver.Methods Twenty-four mice with different degrees of fatty liver underwent CT spectral imaging.CT values of liver parenchyma under mixed X-ray energy and 65 keV,fat concentration based on various basic material pairs (fat/water,fat/io-dine,fat/calcium)and spectral curves were obtained.Liver specimens were obtained to measure the concentration of triglyceride , and HE staining was performed.Correlations between various CT indexes and triglyceride concentration were analyzed.Results Correlation between fat concentration (fat/water pair)and triglyceride (r =0.91 5 )was better than that between CT values on 65 keV and triglyceride (r=-0.858),as well as polychromatic CT values (r=-0.81 6).All the P values were<0.001.Correlations between fat concentrations based on fat/iodine or fat/calcium pairs and triglyceride were relatively low (r=-0.726,-0.660).CT indexes on 1.25 mm slice thickness performed better than those on 2.5 mm.With fatty liver degree increased,the shape of spectral curve changed gradually.Conclusion Liver fat concentration can be measured by CT spectral imaging noninvasively,accurately and quantitatively in a mice model of fatty liver.