CAJ | 학술논문

采用苦参种子为外植体，以 MS 为基本培养基，添加不同浓度的 NAA 和6-BA，进行苦参组培快繁技术研究。结果表明，采用75%乙醇8 s＋0.1%升汞8 min 对苦参种子消毒后，污染率低，易获得无菌材料。 MS 培养基中分别添加0.1 mg/L 、3.0 mg/L 6-BA 和0.5 mg/L NAA、3.0 mg/L 6-BA 的组培苗增殖效果较好，增殖率最高可达120.2%；在1/2 MS 培养基中添加0.1 mg/L 的 NAA，更利于苦参组培苗生根。根长1～2 cm 的无菌苗，在自然光下驯化培养5 d 后移栽至蛭石基质中成活率较高。
채용고삼충자위외식체，이 MS 위기본배양기，첨가불동농도적 NAA 화6-BA，진행고삼조배쾌번기술연구。결과표명，채용75%을순8 s＋0.1%승홍8 min 대고삼충자소독후，오염솔저，역획득무균재료。 MS 배양기중분별첨가0.1 mg/L 、3.0 mg/L 6-BA 화0.5 mg/L NAA、3.0 mg/L 6-BA 적조배묘증식효과교호，증식솔최고가체120.2%；재1/2 MS 배양기중첨가0.1 mg/L 적 NAA，경리우고삼조배묘생근。근장1～2 cm 적무균묘，재자연광하순화배양5 d 후이재지질석기질중성활솔교고。
In this experiment, seeds have been used as explants, MS as the basic medium adding NAA and 6-BA on different concentrations to research rapid propagation technology of Sophora flavescens var. The results shows that the best method of sterilization for seeds is 70% ethanol(8 s)+0.1% HgCl2(8 min) which have the lowest contamination rate and the highest seedling rate. The optimal culture medium for subculture is MS+0.1 mg/L NAA+3.0 mg/L 6-BA and MS+0.5 mg/L NAA+3.0 mg/L 6-BA, the highest multiplication rate is 120.2%, 1/2 MS+0.1 mg/L NAA is the best medium for rooting culture. The tissue culture seedling have 1~2 cm roots could be domesticated with natural light for 5 days, and then transplanted in vermiculite have higher survival rate.