安徽农学通报
安徽農學通報
안휘농학통보
AUHUI AGRICULTURAL SCIENCE BULLETIN
2014年
24期
17-18,129
,共3页
张学江%曾凡松%杨立军%龚双军
張學江%曾凡鬆%楊立軍%龔雙軍
장학강%증범송%양립군%공쌍군
链霉菌%BAC文库%基因组
鏈黴菌%BAC文庫%基因組
련매균%BAC문고%기인조
Streptomyces%BAC library%Genome
实验以抗稻瘟病链霉菌JK-1的基因组DNA为材料构建了其BAC(bacterial artificial chromosome)文库。该文库共有3456个克隆,插入片段在40~100kb,平均插入片段55kb,空载率低于5%,覆盖了链霉菌基因组约17.3倍。抗稻瘟病链霉菌JK-1基因组BAC文库的构建,为进一步研究基因组结构及其功能基因的利用等奠定了基础。
實驗以抗稻瘟病鏈黴菌JK-1的基因組DNA為材料構建瞭其BAC(bacterial artificial chromosome)文庫。該文庫共有3456箇剋隆,插入片段在40~100kb,平均插入片段55kb,空載率低于5%,覆蓋瞭鏈黴菌基因組約17.3倍。抗稻瘟病鏈黴菌JK-1基因組BAC文庫的構建,為進一步研究基因組結構及其功能基因的利用等奠定瞭基礎。
실험이항도온병련매균JK-1적기인조DNA위재료구건료기BAC(bacterial artificial chromosome)문고。해문고공유3456개극륭,삽입편단재40~100kb,평균삽입편단55kb,공재솔저우5%,복개료련매균기인조약17.3배。항도온병련매균JK-1기인조BAC문고적구건,위진일보연구기인조결구급기공능기인적이용등전정료기출。
A genomic bacterial artificial chromosome(BAC)library was constructed using nuclear DNA of Streptomyces JK-1 resistance to Magnaporthe oryzae. The library collected 3 456 recombinant clones carry?ing inserts with sizes ranging from 40kb to 100kb. The average insert size was about 55kb with empty-vec?tor rate less than 5%. This BAC library represented 17.3 equivalents of Streptomyces genome. The BAC li?brary will help further genome and fucntional gene research.