CAJ | 학술논문

【目的】研究斜阳岛附近海域柳珊瑚Anthogorgiacaerulea可培养共生放线菌多样性及其发酵液代谢产物的生物毒活性。【方法】采用纯培养法和基于16 S rRNA基因序列的系统发育分析对从广西斜阳岛附近海域采集的柳珊瑚Anthogorgiacaerulea可培养共生放线菌多样性进行研究，利用卤虫致死法测试其生物毒活性。【结果】从柳珊瑚Anthogorgiacaerulea中分离获得相关可培养放线菌23株，采用16S rRNA基因序列的系统发育分析后发现，这些菌株属于2个亚纲9个科9个属23种。绝大部分菌株都具有一定的生物毒活性，其中10株菌株有较强生物毒活性，1株有显著毒活性。【结论】广西斜阳岛附近海域柳珊瑚Anthogorgia caerulea中存在较为丰富的放线菌多样性，部分菌株具有较强的生物毒活性。
【목적】연구사양도부근해역류산호Anthogorgiacaerulea가배양공생방선균다양성급기발효액대사산물적생물독활성。【방법】채용순배양법화기우16 S rRNA기인서렬적계통발육분석대종엄서사양도부근해역채집적류산호Anthogorgiacaerulea가배양공생방선균다양성진행연구，이용서충치사법측시기생물독활성。【결과】종류산호Anthogorgiacaerulea중분리획득상관가배양방선균23주，채용16S rRNA기인서렬적계통발육분석후발현，저사균주속우2개아강9개과9개속23충。절대부분균주도구유일정적생물독활성，기중10주균주유교강생물독활성，1주유현저독활성。【결론】엄서사양도부근해역류산호Anthogorgia caerulea중존재교위봉부적방선균다양성，부분균주구유교강적생물독활성。
[Objective]To investigate the diversity and biotoxicity of culturable actinomycetes isolated from GorgonianAnthogorgia caerulea collected at the Xieyang island.[Methods]Cul-tivated marine bacteria were isolated from Gorgonian Anthogorgia caerulea collected at the Xieyang island by using conventional culture-dependent method,and then investigate their di-versity by using phylogenetic analysis based on 16S rRNA gene sequence comparisons.Ac-tivities of the crude extract of strains isolated were tested through brine shrimp lethality.[Results]We isolated 23 bacterial strains from the sample in media(2216E,NA and ISP2 ) supplement with seawater.On the basis of morphological and biochemical characteristics,we selected 23 strains to perform a phyloenetic analysis based on 16S rRNA gene sequences. The results showed that there were 2 subclass,9 family,9 genus and 23 species.Activity results showed that the crude extract of 1 0 strains showed obvious brine shrimp lethality.[Conclu-sion]Gorgonian Anthogorgia caerulea collected at the Xieyang island showed high diversity of actinomycetes,and some of them showed obvi- <br> ous biotoxicity against brine shrimp.