CAJ | 학술논문

为研究5-羟色胺（5-HT）受体基因在不同猪种的表达及其与肉质的关系，试验选取淳安花猪与杜长大三元猪各12头，同时饲喂，达上市体重时分别屠宰，测定胴体、肉质性状及肌肉、脂肪中的5-HT受体基因表达量。结果表明：淳安花猪、杜长大三元猪的平均屠宰体重分别为88.1、102.4 kg；淳安花猪的板油率、背膘厚、肌内脂肪等指标均显著高于杜长大三元猪（P＜0.05）；屠宰率、眼肌面积杜长大三元猪显著高于淳安花猪（P＜0.05）。5-HT2A、5-HT7受体基因在2个猪种脂肪组织中的表达量均显著高于肌肉组织（P＜0.05），杜长大三元猪的肌肉中5-HT2A、5-HT7表达量显著低于淳安花猪（P＜0.05），而脂肪中表达量两猪种间差异不显著。5-HT2A、5-HT7受体基因表达量与屠宰肉质指标之间基本呈负相关，尤其是肌肉中的5-HT2A表达量，呈较强负相关（杜长大三元猪）或显著负相关（淳安花猪）。研究初步认为，5-HT受体基因在中外猪种之间存在表达差异，并与屠宰肉质性状具有相关性。
위연구5-간색알（5-HT）수체기인재불동저충적표체급기여육질적관계，시험선취순안화저여두장대삼원저각12두，동시사위，체상시체중시분별도재，측정동체、육질성상급기육、지방중적5-HT수체기인표체량。결과표명：순안화저、두장대삼원저적평균도재체중분별위88.1、102.4 kg；순안화저적판유솔、배표후、기내지방등지표균현저고우두장대삼원저（P＜0.05）；도재솔、안기면적두장대삼원저현저고우순안화저（P＜0.05）。5-HT2A、5-HT7수체기인재2개저충지방조직중적표체량균현저고우기육조직（P＜0.05），두장대삼원저적기육중5-HT2A、5-HT7표체량현저저우순안화저（P＜0.05），이지방중표체량량저충간차이불현저。5-HT2A、5-HT7수체기인표체량여도재육질지표지간기본정부상관，우기시기육중적5-HT2A표체량，정교강부상관（두장대삼원저）혹현저부상관（순안화저）。연구초보인위，5-HT수체기인재중외저충지간존재표체차이，병여도재육질성상구유상관성。
In order to investigate 5-HT receptor genes expression in different breeds and the relationship between meat quality and gene expression,we selected twelve Chun'an spotted pigs and twelve Duroc×Landrace× Yorksire crossbred pigs,uniform feeding and slaughtered at market weight.Carcass characteristics,meat quality traits and 5-HT receptor gene expression in longissimus muscle and subcutaneous fat were detected.The results showed that the average slaughter weight of Chun'an spotted pigs and Duroc×Landrace×Yorksire pigs were 88.1 kg and 102.4 kg respectively.Leaf fat rate,backfat thickness and intramuscular fat of Chun'an spotted pigs were significantly higher than Duroc×Landrace×Yorksire pigs (P<0.05).Slaughter rate,longissimus doris area of Duroc× Landrace×Yorksire pigs were significantly higher than Chun'an spotted pigs(P<0.05).5-HT2A,5-HT7 receptor gene expression levels of subcutaneous fat were significantly higher than longissimus muscle in two breeds (P<0.05);gene expression in longissimus muscle of Duroc ×Landrace ×Yorksire pigs was significantly lower than Chun'an spotted pigs (P<0.05),where as the difference of gene expression in subcutaneous fat was not significant.Gene expression of 5-HT2A and 5-HT7 showed negative correlation with carcass and meat quality traits.The study indicated that the expression of 5-HT receptor gene was different in Chinese and foreign pig breeds.Meanwhile, the expression levels had correlation with carcass and meat quality traits.