CAJ | 학술논문

从多种酸菜水中分离筛选出乳酸菌，利用乳酸菌将谷氨酸转化为GABA的脱羧作用，通过混合酸碱指示剂颜色变化，直观判断谷氨酸脱羧酶(GAD)活性，进而筛选出产GABA乳酸菌菌株BL2，并对其进行初步鉴定；同时，采用纸层析法进行定性分析，确定菌株BL2可将谷氨酸钠转化生成GABA.
종다충산채수중분리사선출유산균，이용유산균장곡안산전화위GABA적탈최작용，통과혼합산감지시제안색변화，직관판단곡안산탈최매(GAD)활성，진이사선출산GABA유산균균주BL2，병대기진행초보감정；동시，채용지층석법진행정성분석，학정균주BL2가장곡안산납전화생성GABA.
LAB Strains were isolated from pickled vegetable juice. Since glutamate acid decarboxylase of LAB can convert monosodium glutamate toγ-aminobutyric acid, color changes of transformation system supplemented with acid-base indicator facilitate screening of GABA-producing LAB. Strain BL2 was isolated and identified. Analysis of paper chromatography suggested the strain’s ability to produce GABA.