CAJ | 학술논문

以苹果抗寒矮化砧木71-3-150的茎尖为外植体,通过研究培养基中不同植物生长调节剂配比对芽苗诱导、增殖和生根的影响,筛选出适合71-3-150砧木茎尖组培快繁的培养基配方。结果表明,以 MS 为基本培养基,附加蔗糖30 g/L、琼脂6 g/L,适宜启动培养的植物生长调节剂为6-BA 1.0 mg/L+NAA 0.05 mg/L；适宜增殖培养的植物生长调节剂为6-BA 1.0 mg/L+NAA 0.05 mg/L,增殖系数可达到每4周4.75倍。以1/2MS (蔗糖15 g/L,琼脂6 g/L)为基本培养基,适宜组培苗生根的植物生长调节剂为 IAA 1.0 mg/L+IBA 0.6 mg/L,生根率达100%,每株苗平均生根9.6条,移栽成活率达90%以上。
이평과항한왜화침목71-3-150적경첨위외식체,통과연구배양기중불동식물생장조절제배비대아묘유도、증식화생근적영향,사선출괄합71-3-150침목경첨조배쾌번적배양기배방。결과표명,이 MS 위기본배양기,부가자당30 g/L、경지6 g/L,괄의계동배양적식물생장조절제위6-BA 1.0 mg/L+NAA 0.05 mg/L；괄의증식배양적식물생장조절제위6-BA 1.0 mg/L+NAA 0.05 mg/L,증식계수가체도매4주4.75배。이1/2MS (자당15 g/L,경지6 g/L)위기본배양기,괄의조배묘생근적식물생장조절제위 IAA 1.0 mg/L+IBA 0.6 mg/L,생근솔체100%,매주묘평균생근9.6조,이재성활솔체90%이상。
Taking the shoot tips of the cold-resistant apple dwarf rootstock 71-3-150 as experimental materials, effects of different plant growth regulators on tissue-cultured seedling induction,multiplication and rooting were researched.The optimal medium formula was selected.The results showed that the basic medium was MS sup-plemented with sugar 30 g/L and agar 6 g/L,and the optimal plant growth regulators of start culture medium that suited the multiplication were 6-BA 1.0 mg/L+NAA 0.05 mg/L.The optimal plant growth regulators of subculture medium were 6-BA 1.0 mg/L+NAA 0.05 mg/L,and the coefficient of multiplication were exceeded 4.75 times every four weeks.For the 1/2 MS supplemented with sugar 15 g/L and agar 6 g/L as basic medium, optimal plant growth regulators of rooting were IAA 1.0 mg/L+ IBA 0.6 mg/L,the rooting rate of shoots was 100 %,and the average number of rooting was 9.6.Then the rooted plantlets were transplanted,and the sur-vival rate was more than 90 %.