岭南现代临床外科
嶺南現代臨床外科
령남현대림상외과
LINGNAN MODERN CLINICS IN SURGERY
2014年
6期
685-687
,共3页
动脉瘤%蛛网膜下腔出血%CD4+CD25+调节性T细胞%细胞因子
動脈瘤%蛛網膜下腔齣血%CD4+CD25+調節性T細胞%細胞因子
동맥류%주망막하강출혈%CD4+CD25+조절성T세포%세포인자
Intracranial aneurysm%Subarachnoid hemorrhage%CD4+CD25+ regulatory T cells%Cytokines
目的：探讨颅内动脉瘤致蛛网膜下腔出血（SAH）患者CD4＋CD25＋调节性T细胞（Treg）的表达及其与分级的关系。方法收集动脉瘤致SAH患者、颅内感染患者及健康人各50例，采用流式细胞术检测外周血Treg百分含量及其细胞因子转化生长因子（TGF-β1）及白介素10（IL-10）的平均荧光强度。比较三组上述三者的差异，并分析三者与动脉瘤致SAH的Hunt分级的相关性。结果动脉瘤组患者Treg、TGF-β1及IL-10均显著低于健康组，且明显低于颅内感染组，差异均有统计学意义，但颅内感染组与对照组无统计学差异。动脉瘤组患者Treg、TGF-β1及IL-10三者当中任意一者与Ⅰ～Ⅴ级当中任意一级均呈负相关性（所得r绝对值均大于0．7，P均小于0．05），三者均与Ⅴ级的相关度最大（P均小于0．001）。结论 Treg、TGF-β1及IL-10可能是颅内动脉瘤患者的一个保护因素，其含量越低则破裂后SAH程度越重，因此三者具有重要临床意义。
目的：探討顱內動脈瘤緻蛛網膜下腔齣血（SAH）患者CD4＋CD25＋調節性T細胞（Treg）的錶達及其與分級的關繫。方法收集動脈瘤緻SAH患者、顱內感染患者及健康人各50例，採用流式細胞術檢測外週血Treg百分含量及其細胞因子轉化生長因子（TGF-β1）及白介素10（IL-10）的平均熒光彊度。比較三組上述三者的差異，併分析三者與動脈瘤緻SAH的Hunt分級的相關性。結果動脈瘤組患者Treg、TGF-β1及IL-10均顯著低于健康組，且明顯低于顱內感染組，差異均有統計學意義，但顱內感染組與對照組無統計學差異。動脈瘤組患者Treg、TGF-β1及IL-10三者噹中任意一者與Ⅰ～Ⅴ級噹中任意一級均呈負相關性（所得r絕對值均大于0．7，P均小于0．05），三者均與Ⅴ級的相關度最大（P均小于0．001）。結論 Treg、TGF-β1及IL-10可能是顱內動脈瘤患者的一箇保護因素，其含量越低則破裂後SAH程度越重，因此三者具有重要臨床意義。
목적：탐토로내동맥류치주망막하강출혈（SAH）환자CD4＋CD25＋조절성T세포（Treg）적표체급기여분급적관계。방법수집동맥류치SAH환자、로내감염환자급건강인각50례，채용류식세포술검측외주혈Treg백분함량급기세포인자전화생장인자（TGF-β1）급백개소10（IL-10）적평균형광강도。비교삼조상술삼자적차이，병분석삼자여동맥류치SAH적Hunt분급적상관성。결과동맥류조환자Treg、TGF-β1급IL-10균현저저우건강조，차명현저우로내감염조，차이균유통계학의의，단로내감염조여대조조무통계학차이。동맥류조환자Treg、TGF-β1급IL-10삼자당중임의일자여Ⅰ～Ⅴ급당중임의일급균정부상관성（소득r절대치균대우0．7，P균소우0．05），삼자균여Ⅴ급적상관도최대（P균소우0．001）。결론 Treg、TGF-β1급IL-10가능시로내동맥류환자적일개보호인소，기함량월저칙파렬후SAH정도월중，인차삼자구유중요림상의의。
Objective To investigate the expression of CD4+CD25+ regulatory T cells (Treg) and its correlation with Hunt’s classification for the patients with subarachnoid hemorrhage caused by intracranial aneurysm. Methods Fifty aneurysm patients with SAH,.50 cases with intracranial infection patients and 50 healthy people were included in this study. The peripheral blood examples were collected to detect the levels of transforming growth factors beta 1 (TGF-beta 1) and interleukin 10 (IL-10) by using flow cytometry. Levels of Tregs, TGF-beta 1 and IL-10 were compared between three groups,.and the correlation between Treg,.TGF-beta 1,.IL-10 and aneurysmal SAH Hunt classification were analyzed. Results The percentage composition of Treg, mean fluorescence intensity of TGF-beta 1 and IL-10 in patients with intracranial aneurysms group were significantly lower than those in healthy group,.and significantly lower than in the intracranial infection patients,.but no statistical difference was found between intracranial infection group and control group. .Any of three levels of Treg,.TGF-beta 1 and IL-10 in aneurysm patients showed a negative correlation with any level of Ⅰ-Ⅴ Hunt classification(r absolute value is greater than 0.7, P<0.05),.in which the maximum correlation showed in Ⅴ level (P<0.001). Conclusion Treg, TGF-beta 1 and IL-10 could be protective factors of patients with intracranial aneurysm ,.and lower expressions of the three factors would worsen the SAH condition.
