岭南现代临床外科
嶺南現代臨床外科
령남현대림상외과
LINGNAN MODERN CLINICS IN SURGERY
2014年
6期
620-623
,共4页
siRNA%真核表达载体%OX40%免疫保护
siRNA%真覈錶達載體%OX40%免疫保護
siRNA%진핵표체재체%OX40%면역보호
siRNA%Eukaryotic expression vector%Immunoprotection
目的：为了探讨真核表达载体介导siRNA在小鼠移植心脏术后的免疫保护作用。方法我们选用次要组织相容性复合体不合的小鼠分成三组作心脏移植并于移植术后第1天、第4天对三组移植小鼠的胸腺分别注射生理盐水、pRNAT-CTL 阴性对照载体和 pRNAT-OX40siRNA。在小鼠移植心脏术后第7天取移植小鼠心脏、血清、脾脏分别进行病理学免疫组化检查、心肌酶谱检测以及OX40mRNA和OX40膜蛋白表达的检测。结果 pRNAT-OX40siRNA组较生理盐水组和阴性对照组，小鼠移植心脏生存时间显著延长，病理改变显著减轻，谷草转氨酶（AST）、肌钙蛋白（Tn）、乳酸脱氢酶（LDH）以及OX40mRNA和OX40膜蛋白表达与空白对照组和阴性对照组相比明显下降，差异具有显著性。结论小鼠次要组织相容性复合体不合的心脏移植中阻断OX40／OX40L免疫通路能够诱导免疫保护效应。
目的：為瞭探討真覈錶達載體介導siRNA在小鼠移植心髒術後的免疫保護作用。方法我們選用次要組織相容性複閤體不閤的小鼠分成三組作心髒移植併于移植術後第1天、第4天對三組移植小鼠的胸腺分彆註射生理鹽水、pRNAT-CTL 陰性對照載體和 pRNAT-OX40siRNA。在小鼠移植心髒術後第7天取移植小鼠心髒、血清、脾髒分彆進行病理學免疫組化檢查、心肌酶譜檢測以及OX40mRNA和OX40膜蛋白錶達的檢測。結果 pRNAT-OX40siRNA組較生理鹽水組和陰性對照組，小鼠移植心髒生存時間顯著延長，病理改變顯著減輕，穀草轉氨酶（AST）、肌鈣蛋白（Tn）、乳痠脫氫酶（LDH）以及OX40mRNA和OX40膜蛋白錶達與空白對照組和陰性對照組相比明顯下降，差異具有顯著性。結論小鼠次要組織相容性複閤體不閤的心髒移植中阻斷OX40／OX40L免疫通路能夠誘導免疫保護效應。
목적：위료탐토진핵표체재체개도siRNA재소서이식심장술후적면역보호작용。방법아문선용차요조직상용성복합체불합적소서분성삼조작심장이식병우이식술후제1천、제4천대삼조이식소서적흉선분별주사생리염수、pRNAT-CTL 음성대조재체화 pRNAT-OX40siRNA。재소서이식심장술후제7천취이식소서심장、혈청、비장분별진행병이학면역조화검사、심기매보검측이급OX40mRNA화OX40막단백표체적검측。결과 pRNAT-OX40siRNA조교생리염수조화음성대조조，소서이식심장생존시간현저연장，병리개변현저감경，곡초전안매（AST）、기개단백（Tn）、유산탈경매（LDH）이급OX40mRNA화OX40막단백표체여공백대조조화음성대조조상비명현하강，차이구유현저성。결론소서차요조직상용성복합체불합적심장이식중조단OX40／OX40L면역통로능구유도면역보호효응。
Objective To value the immunoprotection of transplant heat that was mediated by eukaryotic expression vector of siRNA technique. Methods First, we did mouse heart transplantation using strains representing minor histocompatibility complex antigen mismatches (mHC), and all the experimental animals were divided into three groups. Then we injected respectively sodium chloride ,pRNAT-CTL negative control vector and pRNAT-OX40siRNA into the three groups of mouse thymus at the 1st day and the fourth day after heart transplantation..At the 7th day of post-transplantation, we collected the transplant heart,.blood serum and spleen for pathological examination, besides cardiac muscle zymogram,..RT-PCR and Western blot tests,.respectively..Results The survival time of transplant heart in pRNAT-OX40siRNA was significantly prolonged,..and the transplant heart pathological examination was significantly bettered and lactate dehydrogenase ,.glutamic oxalacetic transaminase, troponin, spleen OX40mRNA expression and OX40 membrane protein expression were significantly lowered compared with those in mice with sodium chloride and pRNAT-CTL negative control vector..Conclusion Our results clearly show that blockade OX40/OX40L pathway has immunoprotection for heart transplantation of mouse with mHC-incompatible.
