CAJ | 학술논문

研究利用特异引物对2份抗病纯合材料（基因型 Mi ／Mi ）和2份感病纯合材料（基因型 mi ／mi）进行 PCR扩增，抗、感材料均产生750 bp 的 PCR 扩增片段，纯合抗病和杂合抗病材料的 PCR 产物存在 Taq I 酶切位点，酶切后分别产生了570 bp 和180 bp 以及750 bp、570 bp 和180 bp 的片段，而感病材料的扩增产物无此酶切位点，酶切后仍为750 bp 的片段。利用该标记对4份番茄杂交种进行检测，其中2份杂交种表现为杂合抗病型，另2份杂交种表现为纯合感病型。利用该标记对其中一个表现为杂合抗病的杂交种50份 F2代单株进行检测，抗感遗传的分离比符合3∶1。说明该分子标记的这一抗病基因属于单基因控制的质量性状遗传位点。
연구이용특이인물대2빈항병순합재료（기인형 Mi ／Mi ）화2빈감병순합재료（기인형 mi ／mi）진행 PCR확증，항、감재료균산생750 bp 적 PCR 확증편단，순합항병화잡합항병재료적 PCR 산물존재 Taq I 매절위점，매절후분별산생료570 bp 화180 bp 이급750 bp、570 bp 화180 bp 적편단，이감병재료적확증산물무차매절위점，매절후잉위750 bp 적편단。이용해표기대4빈번가잡교충진행검측，기중2빈잡교충표현위잡합항병형，령2빈잡교충표현위순합감병형。이용해표기대기중일개표현위잡합항병적잡교충50빈 F2대단주진행검측，항감유전적분리비부합3∶1。설명해분자표기적저일항병기인속우단기인공제적질량성상유전위점。
In this paper,two resistant homozygous lines (Mi /Mi )and two homozygous susceptible lines (Mi /Mi )were amplified by specific primer.Both resistant and susceptible lines produced about 750 bp PCR frag-ment .After digestion with enzyme Taq I ,homozygous resistant genotypes and heterozygous resiatant genotypes could produce fragment about 570 bp,180 bp and 750 bp,570 bp,180 bp,respectively.While the susceptible genotypes still presented 750 bp fragment.Four hybrids were detected using this marker,and the results show that two hybrids are resistant heterozygous and two hybrids are susceptible.The marker also were used to de-tect F2 generation of 50 plants,the Separate ratio of resistant plants and susceptible plants is 3∶1 ,so genetic traits should be controlled by the quality characters.