CAJ | 학술논문

目的：上、下颌骨的发育差异可能是由于两者差异性地表达某些基因而造成的。本文拟对E9.5（胚胎第9.5天）小鼠上、下颌弓差异性表达的基因进行系统的生物信息学分析。方法：从GEO 数据库获取E9.5小鼠上、下颌弓差异性表达的基因，应用DAVID和GeneMANIA数据库分析这些基因之间的相互关系。结果：经过DAVID数据分析，这些在E9.5小鼠上、下颌弓差异性表达的基因被富集到不同的生物学过程或分子功能的子集中，如“转录因子活性”、“系统发育”和“骨骼系统发育”等。其中BTB7B、SOX10、TSHZ2、GSC、GLIS2、MEIS1、CITED2、IRF9、BARX1、MSX1、DLX6、CSRNP1、HEYL、MKX、PITX1和NFIB，这16个基因被富集到“转录因子活性”这一分子功能子集。通过GeneMANIA数据库分析，建立了这16个基因及一些预测基因的分子网络图，显示这些基因存在直接或间接的相互作用。结论：上、下颌骨在发育过程中存在许多差异表达的基因，其中对上、下颌骨特异性发育具有重要调控作用的16个转录因子包括 BTB7B、SOX10、TSHZ2、GSC、GLIS2、MEIS1、CITED2、IRF9、BARX1、MSX1、DLX6、CSRNP1、HEYL、MKX、PITX1和NFIB，它们彼此密切相关且相互作用形成调控网络。在研究上、下颌骨差异性发育的分子调控机制时，需要关注由这些基因形成的调控网络。
목적：상、하합골적발육차이가능시유우량자차이성지표체모사기인이조성적。본문의대E9.5（배태제9.5천）소서상、하합궁차이성표체적기인진행계통적생물신식학분석。방법：종GEO 수거고획취E9.5소서상、하합궁차이성표체적기인，응용DAVID화GeneMANIA수거고분석저사기인지간적상호관계。결과：경과DAVID수거분석，저사재E9.5소서상、하합궁차이성표체적기인피부집도불동적생물학과정혹분자공능적자집중，여“전록인자활성”、“계통발육”화“골격계통발육”등。기중BTB7B、SOX10、TSHZ2、GSC、GLIS2、MEIS1、CITED2、IRF9、BARX1、MSX1、DLX6、CSRNP1、HEYL、MKX、PITX1화NFIB，저16개기인피부집도“전록인자활성”저일분자공능자집。통과GeneMANIA수거고분석，건립료저16개기인급일사예측기인적분자망락도，현시저사기인존재직접혹간접적상호작용。결론：상、하합골재발육과정중존재허다차이표체적기인，기중대상、하합골특이성발육구유중요조공작용적16개전록인자포괄 BTB7B、SOX10、TSHZ2、GSC、GLIS2、MEIS1、CITED2、IRF9、BARX1、MSX1、DLX6、CSRNP1、HEYL、MKX、PITX1화NFIB，타문피차밀절상관차상호작용형성조공망락。재연구상、하합골차이성발육적분자조공궤제시，수요관주유저사기인형성적조공망락。
Objective: The aim of this study was to perform a systematic bioinformatic analysis in differentially expressed genes between the E9.5 mouse maxillary and mandibular arch. Methods: The differentially expressed genes between the E9.5 mouse maxillary and mandibular arch were obtained from the gene expression omnibus database (GEO Datasets). Then the DAVID and GeneMANIA database were used to analyse the relationships during these genes. Results: These differentially expressed genes between the E9.5 mouse maxillary and mandibular arch could be enriched into different "biological process" and "molecular function" subgroups, based on the analysis of DAVID database, including "transcription factor activity", "system development", "skeleton system development", etc. Sixteen genes, including BTB7B, SOX10, TSHZ2, GSC, GLIS2, MEIS1, CITED2, IRF9, BARX1, MSX1, DLX6, CSRNP1, HEYL, MKX, PITX1 and NFIB, were enriched into the "transcription factor activity" subgroup, and these genes were clearly revealed to have direct or indirect interaction with each other based on the analysis of GeneMANIA database. Conclusions: There were many differentially expressed genes during the maxillary and mandibular development process, and sixteen transcription factors,including BTB7B, SOX10, TSHZ2, GSC, GLIS2, MEIS1, CITED2, IRF9, BARX1, MSX1, DLX6, CSRNP1, HEYL, MKX, PITX1 and NFIB, played crucial roles during the differential development of the jaws and closely related to each other. The molecular networks composed by these genes involved in the development of jaws should be paid special attention when study the molecular mechanism of differentially developmental maxilla and mandible.