CAJ | 학술논문

目的：探讨 menin 蛋白对髓样分化因子88(myeloid differentiation factor 88, MyD88)基因表达的调控作用。方法在 Men1基因敲除的小鼠 mef (Men1-/- mef)细胞中,转染可以过表达 menin 蛋白的 me-nin-PCI-NEO 质粒,运用 Western 印迹技术检测 menin 对 MyD88蛋白表达的影响。构建包含 MyD88启动子的报告基因质粒,在 Men1-/- mef 细胞中,将 MyD88启动子质粒和 menin-PCI-NEO 质粒或其阴性对照 PCI-NEO 共转染,利用荧光报告基因系统分析 menin 对 MyD88基因启动子活性的影响。结果转染 menin-PCI-NEO 质粒后, menin 蛋白表达升高的同时, MyD88蛋白的表达减少。在荧光报告基因系统中,转染 menin-PCI-NEO 质粒后 MyD88基因启动子活性被抑制。结论 menin 蛋白可以下调 MyD88蛋白的表达,这种作用可能通过抑制 MyD88基因的转录而实现。
목적：탐토 menin 단백대수양분화인자88(myeloid differentiation factor 88, MyD88)기인표체적조공작용。방법재 Men1기인고제적소서 mef (Men1-/- mef)세포중,전염가이과표체 menin 단백적 me-nin-PCI-NEO 질립,운용 Western 인적기술검측 menin 대 MyD88단백표체적영향。구건포함 MyD88계동자적보고기인질립,재 Men1-/- mef 세포중,장 MyD88계동자질립화 menin-PCI-NEO 질립혹기음성대조 PCI-NEO 공전염,이용형광보고기인계통분석 menin 대 MyD88기인계동자활성적영향。결과전염 menin-PCI-NEO 질립후, menin 단백표체승고적동시, MyD88단백적표체감소。재형광보고기인계통중,전염 menin-PCI-NEO 질립후 MyD88기인계동자활성피억제。결론 menin 단백가이하조 MyD88단백적표체,저충작용가능통과억제 MyD88기인적전록이실현。
Objective To explore the role of menin, the protein product of the Men1 gene, in the regulation of myeloid differentiation factor 88 (MyD88) gene expression. Methods The plas-mid menin-PCI-NE0 that can overexpress menin protein was transfected into Men1-/ - mef cells of mouse. Western blotting was performed to examine the effect of menin on the expression of MyD88. Reporter gene vectors with MyD88 promoter were constructed and co-transfected into Men1-/ -mef cells along with menin-PCI-NEC and negative control PCI-NEO, and the influence of menin on the transcription activity of MyD88 promoter was analyzed by dual-luciferase reporter gene assay sys-tem. Results Western blotting showed that menin was increased while MyD88 decreased after trans-fection of menin-PCI-NEO plasmids. Additionally, dual-luciferase reporter gene assay system re-vealed that the activity of the MyD88 gene promoter was significantly inhibited after the transfec-tion. Conclusion Menin may inhibit the expression of MyD88 protein by inhibiting the transcription of MyD88 gene.