CAJ | 학술논문

目的：观察牛蒡苷元对增生性瘢痕(hypertrophic scar)形成的作用,并探讨其抑制增生性瘢痕形成的机制。方法新西兰大耳兔25只,将其分为对照组( A 组),模型组(B 组),牛蒡苷元治疗组(0.5 mg/ ml)(C 组),牛蒡苷元治疗组(2 mg/ ml)(D 组),牛蒡苷元治疗组(6 mg/ ml)(E 组),在兔耳腹侧面建立增生性瘢痕模型,术后按组别进行相应处理,观察创面愈合和瘢痕的增生情况。用药后在第6周取材,进行 HE 染色,天狼猩红染色和 Masson 染色,并用 Western 印迹检测 MMP-2, MMP-9表达情况。结果 HE 染色,天狼星红染色和 Masson 染色结果表明,牛蒡苷元治疗组(2 mg/ ml)可以明显抑制增生性瘢痕的形成。与模型组相比,牛蒡苷元治疗组的瘢痕较平坦,成纤维细胞的数量减少,胶原的密度降低。并且 Western 印迹的结果表明,牛蒡苷元治疗组(2 mg/ ml)能够使 MMP-2, MMP-9表达降低。结论牛蒡苷元能有效抑制增生性瘢痕的发展,其机制可能是通过下调 MMP-2和 MMP-9的表达。牛蒡苷元可能具有治疗增生性瘢痕的作用。
목적：관찰우방감원대증생성반흔(hypertrophic scar)형성적작용,병탐토기억제증생성반흔형성적궤제。방법신서란대이토25지,장기분위대조조( A 조),모형조(B 조),우방감원치료조(0.5 mg/ ml)(C 조),우방감원치료조(2 mg/ ml)(D 조),우방감원치료조(6 mg/ ml)(E 조),재토이복측면건립증생성반흔모형,술후안조별진행상응처리,관찰창면유합화반흔적증생정황。용약후재제6주취재,진행 HE 염색,천랑성홍염색화 Masson 염색,병용 Western 인적검측 MMP-2, MMP-9표체정황。결과 HE 염색,천랑성홍염색화 Masson 염색결과표명,우방감원치료조(2 mg/ ml)가이명현억제증생성반흔적형성。여모형조상비,우방감원치료조적반흔교평탄,성섬유세포적수량감소,효원적밀도강저。병차 Western 인적적결과표명,우방감원치료조(2 mg/ ml)능구사 MMP-2, MMP-9표체강저。결론우방감원능유효억제증생성반흔적발전,기궤제가능시통과하조 MMP-2화 MMP-9적표체。우방감원가능구유치료증생성반흔적작용。
Objective To investigate the effects of arctigenin on the formation of hypertrophic scars and the possible mechanism. Methods Twenty-five rabbits were randomly divided into five groups: control group, model group, 0. 5 mg / mL arctigenin group, 2 mg / mL arctigenin group and 6 mg / mL arctigenin group. Hypertrophic scars were induced on the ventral surface of rabbit ears and treated with arctigenin at different doses. The wound healing and hyperplasia of the scars were ob-served. The scar tissues were removed for histopathological detection with HE staining, Sirius red staining and Masson staining and for detection of expression of MMP-2 and MMP-9 with Western blotting 6 weeks after treatment. Results HE staining, Sirius red staining and Masson staining showed that arctigenin (2 mg / mL) could significantly inhibit the hyperplasia of the scars. The scars were flatter, the number of fibroblasts and the density of collagen were less in the arctigenin-treated groups than in the model group. Western blotting showed that the expression levels of MMP-2 and MMP-9 were significantly decreased in 2 mg / mL arctigenin group. Conclusion Arctigenin may prevent the formation of hypertrophic scars by reducing the expression of MMP-2, MMP-9 and it may be used to treat hyperplastic scars.