腐植酸
腐植痠
부식산
HUMIC ACID
2014年
6期
28-31
,共4页
S.Karim%M.Aoyama%刘毓芳%吴云彬%赵瑞华%张彩凤(译)
S.Karim%M.Aoyama%劉毓芳%吳雲彬%趙瑞華%張綵鳳(譯)
S.Karim%M.Aoyama%류육방%오운빈%조서화%장채봉(역)
荧光%腐植酸%腐殖化程度%制备电泳%聚丙烯酰胺凝胶电泳(PAGE)%尿素
熒光%腐植痠%腐殖化程度%製備電泳%聚丙烯酰胺凝膠電泳(PAGE)%尿素
형광%부식산%부식화정도%제비전영%취병희선알응효전영(PAGE)%뇨소
fluorescence%humic acid%humification degree%preparative electrophoresis%polyacrylamide gel electrophoresis(PAGE)%urea
采用制备型电泳体系在高浓度的尿素存在下进行聚丙烯酰胺凝胶电泳分离水溶性腐植酸中的不同成分。在聚丙烯酰胺凝胶电泳中，根据水溶性腐植酸中深色成分分子大小的不同能够将它们彼此分离开来，同时还可以通过酸沉淀的方法将这些深色物质重新回收。水溶性腐植酸中大部分的荧光物质分子尺寸都很小，这使得它们可以溶于酸，并且被酸溶解的荧光物质可以通过吸附在DAX-8树脂上而重新获得。但是，水溶性腐植酸中的非荧光物质则会在电泳过程中丢失。而那些被认为是小分子的荧光物质则会通过氢键的断裂和7M尿素的疏水作用得到分离。漫反射傅里叶变换红外光谱的测量结果表明腐植酸中不同成分的化学性质是不同的。而实验结果表明在高浓度尿素存在下聚丙烯酰胺凝胶电泳是分离和收集水溶性腐植酸中各组分的一种很有用的方法。
採用製備型電泳體繫在高濃度的尿素存在下進行聚丙烯酰胺凝膠電泳分離水溶性腐植痠中的不同成分。在聚丙烯酰胺凝膠電泳中，根據水溶性腐植痠中深色成分分子大小的不同能夠將它們彼此分離開來，同時還可以通過痠沉澱的方法將這些深色物質重新迴收。水溶性腐植痠中大部分的熒光物質分子呎吋都很小，這使得它們可以溶于痠，併且被痠溶解的熒光物質可以通過吸附在DAX-8樹脂上而重新穫得。但是，水溶性腐植痠中的非熒光物質則會在電泳過程中丟失。而那些被認為是小分子的熒光物質則會通過氫鍵的斷裂和7M尿素的疏水作用得到分離。漫反射傅裏葉變換紅外光譜的測量結果錶明腐植痠中不同成分的化學性質是不同的。而實驗結果錶明在高濃度尿素存在下聚丙烯酰胺凝膠電泳是分離和收集水溶性腐植痠中各組分的一種很有用的方法。
채용제비형전영체계재고농도적뇨소존재하진행취병희선알응효전영분리수용성부식산중적불동성분。재취병희선알응효전영중，근거수용성부식산중심색성분분자대소적불동능구장타문피차분리개래，동시환가이통과산침정적방법장저사심색물질중신회수。수용성부식산중대부분적형광물질분자척촌도흔소，저사득타문가이용우산，병차피산용해적형광물질가이통과흡부재DAX-8수지상이중신획득。단시，수용성부식산중적비형광물질칙회재전영과정중주실。이나사피인위시소분자적형광물질칙회통과경건적단렬화7M뇨소적소수작용득도분리。만반사부리협변환홍외광보적측량결과표명부식산중불동성분적화학성질시불동적。이실험결과표명재고농도뇨소존재하취병희선알응효전영시분리화수집수용성부식산중각조분적일충흔유용적방법。
To separate Humic Acid(HAs) into their constituents, Polyacrylamide Gel Electrophoresis(PAGE) was carried out in the presence of 7 M urea using a preparative electrophoresis system. Upon PAGE the dark-colored constituents of HAs were separated by their molecular sizes and recovered by precipitation with acid. Considerable parts of the lfuores-cent constituents of HAs with the lowest molecular size were rendered soluble in acid and the acid soluble lfuorescent constituents were recovered by adsorption onto DAX-8 resin. However, the colorless constituents of HAs were lost during the electrophoresis. These constituents were considered as the low molecular sizes constituents separated due to disruption of the hydrogen bonding and hydrophobic interaction by 7 M urea. The measurement of diffuse relfection infrared Fourier transform spectra indicated that the chemical properties of HA constituents were different from each other. The results show that preparative PAGE in the presence of concentrated urea is a useful method for fractionating and collecting the constituents of HAs.
