CAJ | 학술논문

为了探索花粉管生长调控的机制，本研究利用花粉管模型，结合超微结构观察测量花粉管的内径，壁厚，囊泡大小，首次定量分析了花粉管生长速度变化过程中的胞吞和胞吐速率变化。结果发现在0?01％外钙浓度下花粉管平均生长速率为（8?74±1?83）μm／min，胞吐速率9043个／min，胞吞速率344749个／min；在0?3％浓度下花粉管均生长速率为（3?47±0?93）μm／min，胞吐速率12873个／min，胞吞速率517738个／min；这证明了花粉管生长速度与胞吞胞吐速率不成直接的正比关系。其次通过细胞壁成分的荧光标记观察，还发现在0?3％的外钙浓度下，花粉管细胞壁中胼胝质明显增加，顶端生长点细胞壁内纤维素和酸性果胶略有增多。这表明细胞壁成分的变化对于花粉管生长速度的调节有着重要的影响。
위료탐색화분관생장조공적궤제，본연구이용화분관모형，결합초미결구관찰측량화분관적내경，벽후，낭포대소，수차정량분석료화분관생장속도변화과정중적포탄화포토속솔변화。결과발현재0?01％외개농도하화분관평균생장속솔위（8?74±1?83）μm／min，포토속솔9043개／min，포탄속솔344749개／min；재0?3％농도하화분관균생장속솔위（3?47±0?93）μm／min，포토속솔12873개／min，포탄속솔517738개／min；저증명료화분관생장속도여포탄포토속솔불성직접적정비관계。기차통과세포벽성분적형광표기관찰，환발현재0?3％적외개농도하，화분관세포벽중변지질명현증가，정단생장점세포벽내섬유소화산성과효략유증다。저표명세포벽성분적변화대우화분관생장속도적조절유착중요적영향。
In order to decipher the mechanism underlying pollen tube growth, exocytosis/endocytosis rates of Lili pollen tubes cultured in medium with excellular calcium at different concentrations were first quantitatively compared base on an algorithm using growth velocities of pollen tubes, widths of pollen tube lumens, thicknesses of cell walls, and diameters of endocytosis and exocytosis vesicles. The results showed that pollen tubes cultured in medium with 0?01% calcium grew at mean velocity of (8?74 ± 1?83)μm/min, which required exocytosis rate of 9 043 events/min and endocytosis rate of 344 749 events /min; while as in 0?3% calcium, pollen tubes grew at mean velocity of (3?47 ± 0?93)μm/min, which required exocytosis rate of 12 873 events/min and endocytosis rate of 517 738 events /min, suggesting there is no certain positive correlation between growth velocity of pollen tube and exocytosis/endocytosis rate. Moreover, fluorescence labeling of cell wall components revealed that, compared with 0?01% calcium in medium, 0?03% calcium obviously induced callose in cell walls along whole pollen tubes, and caused a bit of increase of cellulose and acidic pectin at apical cell walls, indicating that cell wall components played important roles in the regulation of growth velocity of pollen tube.