CAJ | 학술논문

阿魏酸是导致很多作物产生连作障碍的自毒物质。筛选出1株高效降解阿魏酸的细菌，初步鉴定为葡萄球菌属，命名为A WS4B ，研究了A WS4B对阿魏酸的降解特征，探讨了其降解途径。结果表明，当无机盐培养基中阿魏酸的浓度为100 mg／L 时，菌株AWS4B 72 h可降解99.97％。降解过程符合一级动力学模型，反应的活化能 Ea 为19.88 kJ／mol ，降解方程常数 k0为3.26×10－4，得出了菌株AWS4B降解阿魏酸的预测模型方程。AWS4B降解阿魏酸的底物来源比较广泛。菌株AWS4B对阿魏酸降解的可能途径是非氧化脱羧形成香草醛，再氧化形成香草酸，脱甲基后形成原儿茶酸，最后原儿茶酸苯环裂解后分解为水和二氧化碳，最终实现阿魏酸的降解。
아위산시도치흔다작물산생련작장애적자독물질。사선출1주고효강해아위산적세균，초보감정위포도구균속，명명위A WS4B ，연구료A WS4B대아위산적강해특정，탐토료기강해도경。결과표명，당무궤염배양기중아위산적농도위100 mg／L 시，균주AWS4B 72 h가강해99.97％。강해과정부합일급동역학모형，반응적활화능 Ea 위19.88 kJ／mol ，강해방정상수 k0위3.26×10－4，득출료균주AWS4B강해아위산적예측모형방정。AWS4B강해아위산적저물래원비교엄범。균주AWS4B대아위산강해적가능도경시비양화탈최형성향초철，재양화형성향초산，탈갑기후형성원인다산，최후원인다산분배렬해후분해위수화이양화탄，최종실현아위산적강해。
Ferulic acid (FA ) was one of important auto‐toxic chemicals leading to continuous cropping obstacle for many crops. A bacterium strain AWS4B was screened out and identified preliminarily as Staphylococcus sp. ,named as AWS4B. The degradation characteristics was studied and the pathway of the degradation was discussed. The results showed that the degradation rate reached 99.97% in 72 h when the initial FA concentration in the inorganic salt urbane liquor was 100 mg/L. The degradation of FA followed first‐order reaction kinetics model and the thermal degradation activation energy was 19.88 kJ/molThe rate constant(k0 )was 3.26 × 10-4 and equation for strain AWS4B prediction model was proposed in this paper. Many compounds provide strain AWS4B with carbon and enery. The influence of different nutrient substrates added in the degradation experiments was also investigated. Degradation pathway was likely to be that FA was degraded into Vanillin , Vanillic acid and protocatechuic acid through non‐oxidative decarboxylation ,oxidation and demethylation. The protocatechuic acid was depredated through benzene ring cleavage and water and carbon dioxide were produced finally and FA was degraded by strain AWS 4B.