中国西部科技
中國西部科技
중국서부과기
SCIENCE AND TECHNOLOGY OF WEST CHINA
2014年
12期
68-71
,共4页
UPLC-MS/MS%茶碱%中成药%保健食品
UPLC-MS/MS%茶堿%中成藥%保健食品
UPLC-MS/MS%다감%중성약%보건식품
UPLC-MS/MS%Theophylline%Chinese Patent Medicine%Healthcare Products
为建立准确、灵敏的UPLC-MS/MS方法直接测定止咳平喘类中药制剂及保健食品中非法添加的茶碱的含量，采用Agilent ZORBAX-SB-C18柱为分析柱，以甲醇-0.1%甲酸铵(30：70)为流动相，检测波长270nm，扫描方式采用全扫描一级质谱、全扫描二级质谱以及MRM模式定量，质量数范围100～300。结果显示UPLC-MS/MS方法测定茶碱的检出限为scan模式下50ng/ml、MRM模式下0.05ng/ml；茶碱的定量限为MRM模式下0.1ng/ml，在0.1ng/ml～2.0ng/ml范围内浓度与峰面积呈良好的线性关系(r=0.9975)。该方法建立的系统能够灵敏、准确地测定止咳平喘类中药制剂及保健食品中茶碱含量。
為建立準確、靈敏的UPLC-MS/MS方法直接測定止咳平喘類中藥製劑及保健食品中非法添加的茶堿的含量，採用Agilent ZORBAX-SB-C18柱為分析柱，以甲醇-0.1%甲痠銨(30：70)為流動相，檢測波長270nm，掃描方式採用全掃描一級質譜、全掃描二級質譜以及MRM模式定量，質量數範圍100～300。結果顯示UPLC-MS/MS方法測定茶堿的檢齣限為scan模式下50ng/ml、MRM模式下0.05ng/ml；茶堿的定量限為MRM模式下0.1ng/ml，在0.1ng/ml～2.0ng/ml範圍內濃度與峰麵積呈良好的線性關繫(r=0.9975)。該方法建立的繫統能夠靈敏、準確地測定止咳平喘類中藥製劑及保健食品中茶堿含量。
위건립준학、령민적UPLC-MS/MS방법직접측정지해평천류중약제제급보건식품중비법첨가적다감적함량，채용Agilent ZORBAX-SB-C18주위분석주，이갑순-0.1%갑산안(30：70)위류동상，검측파장270nm，소묘방식채용전소묘일급질보、전소묘이급질보이급MRM모식정량，질량수범위100～300。결과현시UPLC-MS/MS방법측정다감적검출한위scan모식하50ng/ml、MRM모식하0.05ng/ml；다감적정량한위MRM모식하0.1ng/ml，재0.1ng/ml～2.0ng/ml범위내농도여봉면적정량호적선성관계(r=0.9975)。해방법건립적계통능구령민、준학지측정지해평천류중약제제급보건식품중다감함량。
To establish a rapid, accurate UPLC -MS/MS method for the determination of theophylline, which were illegally added into traditional Chinese medicines and healthcare products. Method: The analytes were separated on a Agilent ZORBAX-SB-C18 column with methanol -0.1% ammonium formate (30:70) as mobile phase, detecting wavelength at 270nm, in three scan modes including full scan MS, full scan MS2, and MRM model. Results: A good resolution was obtained for theophylline under the UPLC and MS conditions. The determination limits were in the range of 0.1ng/ml~2.0ng/ml. Conclusion:The method is simple, rapid, accurate and highly sensitive, which can be used for the determination of traditional Chinese medicine and healthcare products mixed with theophylline.
