CAJ | 학술논문

目的：摸索X-GVHD模型的合适小鼠品系，建立稳定的“人-鼠”X-GVHD模型。方法：选取了裸鼠、NOD／SCID经亚致死剂量γ射线全身照射后，腹腔输注健康人外周血单核细胞（ PBMC）建立异种X-GVHD模型。通过检测人T细胞在模型鼠外周血、组织、器官中的浸润情况等指标（采用流式细胞术和免疫组化技术），比较两种模型鼠中人免疫细胞的浸润率和各组生存时间，从而确定建立X-GVHD模型的合适小鼠品系，并摸索人PBMC的输注途径和合适细胞量，以及观察免疫细胞表型变化与功能的最佳时间窗口。结果：NOD／SCID鼠更适合诱导“人-鼠”X-GVHD模型的小鼠；腹腔输注途径和尾静脉输注途径对建立“人-鼠”X-GVHD模型无明显差异；腹腔输注5×107以上人PBMC可以成功建立“人-鼠”X-GVHD模型。在采用优化后的实验条件建立模型中，监测人T细胞表型动态变化与功能的最佳时间窗口为7～11 d；模型鼠的平均生存时间为（14.16±1.77）d。结论：NOD／SCID鼠通过腹腔注射5×107以上人PBMC可以成功建立“人-鼠”X-GVHD模型，7～11 d为监测人T细胞表型动态变化与功能的最佳时间。
목적：모색X-GVHD모형적합괄소서품계，건립은정적“인-서”X-GVHD모형。방법：선취료라서、NOD／SCID경아치사제량γ사선전신조사후，복강수주건강인외주혈단핵세포（ PBMC）건립이충X-GVHD모형。통과검측인T세포재모형서외주혈、조직、기관중적침윤정황등지표（채용류식세포술화면역조화기술），비교량충모형서중인면역세포적침윤솔화각조생존시간，종이학정건립X-GVHD모형적합괄소서품계，병모색인PBMC적수주도경화합괄세포량，이급관찰면역세포표형변화여공능적최가시간창구。결과：NOD／SCID서경괄합유도“인-서”X-GVHD모형적소서；복강수주도경화미정맥수주도경대건립“인-서”X-GVHD모형무명현차이；복강수주5×107이상인PBMC가이성공건립“인-서”X-GVHD모형。재채용우화후적실험조건건립모형중，감측인T세포표형동태변화여공능적최가시간창구위7～11 d；모형서적평균생존시간위（14.16±1.77）d。결론：NOD／SCID서통과복강주사5×107이상인PBMC가이성공건립“인-서”X-GVHD모형，7～11 d위감측인T세포표형동태변화여공능적최가시간。
Objective:To explore the suitable mouse strains,and establish stable "human-mice"X-GVHD model.Methods:This study selected the Nude Mice and NOD/SCID, and gave them sublethal dose of γ-ray irradiation whole body , and then intraperitoneal transplanted human peripheral blood mononuclear cells ( PBMC) to establish xenogeneic acute graft-versus-host disease model.By detection of human T cells in the mice′s tail venous blood,tissues,organs of the infiltration and other indicators (By flow cy-tometry and immunohistochemistry ) ,we compared the human immune cell infiltration rates in the two mouse model and recorded the survival time.Finally,we determined the appropriate strains of mice to establish X-GVHD.Optimization means were transfer ways and the appropriate amount of human PBMC ,and the best time to observe the changes of T cell phenotype and function.Results:The NOD/SCID mouse was more suitable for inducing "human-mouse"X-GVHD model,and there were no significant differences between intrap-eritoneal injection and intravenous injection.Transfer human PBMC more than 5 ×107 can establish "human-mouse"X-GVHD model.Using the optimized experimental conditions to establish the "human-mouse"X-GVHD model,we found the 7-11 days was the best time to observe the changes of T cell phenotype and function ,and the average survival time was(14.16±1.77)days.Conclusion:"Human-mice"X-GVHD model can be successfully established by intraperitoneal injection of 5×107 human PBMC into NOD/SCID, and the best time to observe the changes of T cell phenotype and function is between 7-11 days.