CAJ | 학술논문

目的：探讨MSCs上清对雷公藤甲素损害卵巢颗粒细胞的缓解作用及机制。方法：用CCK-8法分析了雷公藤甲素对KGN活力的影响；用混合酶消化法分离脐带来源的MSCs，并用流式细胞术对其表型进行了鉴定；收集MSCs上清，用CCK-8法及PI法检测MSCs上清对雷公藤甲素损伤的KGN活力及周期的影响；用Real-time PCR 法对周期调节相关基因CDKN1A的表达进行检测。结果：雷公藤甲素能够通过抑制KGN活力及阻滞KGN在S期抑制其生长；MSCs上清不影响正常KGN的增殖和周期，但可提高雷公藤甲素损伤的KGN细胞的活力，缓解雷公藤甲素引起的S期阻滞，抑制雷公藤甲素对KGN的CDKN1A表达的异常上调。结论：MSCs上清可能减轻雷公藤甲素对KGN细胞的损伤作用。
목적：탐토MSCs상청대뢰공등갑소손해란소과립세포적완해작용급궤제。방법：용CCK-8법분석료뢰공등갑소대KGN활력적영향；용혼합매소화법분리제대래원적MSCs，병용류식세포술대기표형진행료감정；수집MSCs상청，용CCK-8법급PI법검측MSCs상청대뢰공등갑소손상적KGN활력급주기적영향；용Real-time PCR 법대주기조절상관기인CDKN1A적표체진행검측。결과：뢰공등갑소능구통과억제KGN활력급조체KGN재S기억제기생장；MSCs상청불영향정상KGN적증식화주기，단가제고뢰공등갑소손상적KGN세포적활력，완해뢰공등갑소인기적S기조체，억제뢰공등갑소대KGN적CDKN1A표체적이상상조。결론：MSCs상청가능감경뢰공등갑소대KGN세포적손상작용。
Objective:To explore the ameliorative effect and machanism of MSCs conditioned medium on the ovarian granulosa cells damage induced by triptolide.Methods: Cell Counting Kit 8 assay was used to examine the cell vitality of KGNs with the treatment of triptolide.The mixed enzyme digestion method were used for the isolation of human umbilical cord mesenchymal stem cells (MSCs),and flow cytometry was used for the subsequent immunotype identification.MSCs conditioned medium was collected ,and Cell Counting Kit 8 assay and PI staining was used to analyse the effect of MSCs conditioned medium on the cell vitality and cell cycle distri -bution of triptolide-damaged KGN.Real-time PCR method was used to examine the expression of cell cycle related gene CDKN1A.Results:Triptolide can inhibit KGN cell growth with the inhibition of cell vitality and cell cycle of KGN.MSCs conditioned medium did not influence the proliferation and cell cycle of normal KGN , but improved the triptolide-induced vitality inhibition and extent of S-phase arrest, and inhibit the abnormal up-regulation of CDKN1A in KGN.Conclusion: MSCs conditioned medium ameliorated the KGN cell damage induced by triptolide.