CAJ | 학술논문

为降低再造烟叶中还原糖含量，模拟生产中烟草萃取液减压浓缩、涂布烘烤等步骤，在萃取液中添加适量氨基酸，以氯化铁（FeCl3）为催化剂进行美拉德反应；采用高效液相色谱法测定还原糖含量变化，研究了不同种类氨基酸及其用量、催化剂用量、萃取液pH以及浓缩与涂布烘烤条件对降低还原糖含量的影响。结果表明：①当萃取液中还原糖∶氨基酸（摩尔比）=1∶0.5时，反应体系中Fe3+含量为0.01 mmol/L ，用2.5 mol/L K2CO3调节pH至6.2左右，模拟生产条件进行减压浓缩和涂布烘烤，可降低再造烟叶中还原糖含量。其中赖氨酸反应效果最好，相对于空白，再造烟叶中还原糖含量降低10.6%；甘氨酸和脯氨酸分别降低9.1%和6.0%。②与空白对照感官评价结果相比，制得的再造烟叶刺激性有所降低，余味改善，其中甘氨酸处理的再造烟叶感官评价效果最好，脯氨酸处理的效果与空白对照样品相似。
위강저재조연협중환원당함량，모의생산중연초췌취액감압농축、도포홍고등보취，재췌취액중첨가괄량안기산，이록화철（FeCl3）위최화제진행미랍덕반응；채용고효액상색보법측정환원당함량변화，연구료불동충류안기산급기용량、최화제용량、췌취액pH이급농축여도포홍고조건대강저환원당함량적영향。결과표명：①당췌취액중환원당∶안기산（마이비）=1∶0.5시，반응체계중Fe3+함량위0.01 mmol/L ，용2.5 mol/L K2CO3조절pH지6.2좌우，모의생산조건진행감압농축화도포홍고，가강저재조연협중환원당함량。기중뢰안산반응효과최호，상대우공백，재조연협중환원당함량강저10.6%；감안산화포안산분별강저9.1%화6.0%。②여공백대조감관평개결과상비，제득적재조연협자격성유소강저，여미개선，기중감안산처리적재조연협감관평개효과최호，포안산처리적효과여공백대조양품상사。
In order to decrease the content of reducing sugar in reconstituted tobacco (RT), Maillard reaction was brought about by adding amino acids in tobacco extract and FeCl3 as catalyst, going through extract concentration under reduced pressure, applying and drying steps analogous to real RT production. The content of reducing sugar was determined with HPLC, and the effects of different amino acids, addition rates of amino acids and catalyst, pH of extract, and conditions of extract concentrating, applying and drying on the reducing sugar content were investigated. The results showed that: 1) When reducing sugar/amino acid ratio in extract was 1∶0.5 (mole ratio), Fe3+ concentration 0.01 mmol/L, pH adjusted to about 6.2 by 2.5 mol/L K2CO3, the content of reducing sugar in RT could be decreased subject to simulated concentrating, applying and drying. Comparing with the control, the reducing sugar content in RT treated with lysine, glycine and proline decreased by 10.6%, 9.1% and 6.0%, respectively. 2) Comparing with the control, the irritancy of the RT treated with amino acids decreased to a certain extent, the aftertaste was improved; and the glycine-treated RT offered the best smoking quality.