CAJ | 학술논문

目的：探讨RhoE与EGFR在食管鳞状细胞癌中的表达及其关联性，并分析其临床意义。方法运用快捷免疫组化方法对我院2011年1月至2012年1月收集的食管鳞状细胞癌癌组织及相应的癌旁组织59例进行检测，测定RhoE与EGFR的表达情况，运用统计学方法结合患者其他指标进行分析。结果①食管鳞癌癌旁正常组织中RhoE蛋白的阳性表达灰度值是52．131±3．682，食管鳞癌组织中为37．115±4．314，二者差异有统计学意义（P＜0．001）。②食管鳞癌癌旁正常组织中EGFR蛋白的阳性表达灰度值是33．956±3．716，食管鳞癌组织中为48．168±2．945，二者差异有统计学意义（P＜0．05）。③二者表达水平情况与肿瘤的临床分期、分化程度、淋巴转移以及有无浸润都有密切关系（P＜0．001），与肿瘤的大体分型、大小和部位无关（P＞0．05）。④RhoE与EGFR在食管鳞癌组织中的表达呈负相关（P＜0．01）。结论 EGFR基因的表达水平在食管鳞癌中是增高的，而RhoE则是降低的；并且二者呈负相关，与肿瘤的分化程度、有无浸润、淋巴的转移以及临床分期等密切相关，这提示着EGFR的高表达或许参与了肿瘤的发生与发展，对二者的表达情况进行检测有助于我们了解食管癌的生物学行为以及评估预后。
목적：탐토RhoE여EGFR재식관린상세포암중적표체급기관련성，병분석기림상의의。방법운용쾌첩면역조화방법대아원2011년1월지2012년1월수집적식관린상세포암암조직급상응적암방조직59례진행검측，측정RhoE여EGFR적표체정황，운용통계학방법결합환자기타지표진행분석。결과①식관린암암방정상조직중RhoE단백적양성표체회도치시52．131±3．682，식관린암조직중위37．115±4．314，이자차이유통계학의의（P＜0．001）。②식관린암암방정상조직중EGFR단백적양성표체회도치시33．956±3．716，식관린암조직중위48．168±2．945，이자차이유통계학의의（P＜0．05）。③이자표체수평정황여종류적림상분기、분화정도、림파전이이급유무침윤도유밀절관계（P＜0．001），여종류적대체분형、대소화부위무관（P＞0．05）。④RhoE여EGFR재식관린암조직중적표체정부상관（P＜0．01）。결론 EGFR기인적표체수평재식관린암중시증고적，이RhoE칙시강저적；병차이자정부상관，여종류적분화정도、유무침윤、림파적전이이급림상분기등밀절상관，저제시착EGFR적고표체혹허삼여료종류적발생여발전，대이자적표체정황진행검측유조우아문료해식관암적생물학행위이급평고예후。
Objective To explore the expression,correlation and significance of RhoE and EGFRin esophageal squamous cell carcinoma(ESCC).Method From Jan 2011 to Jan 2012,a total of 59 casesof ESCC and adjacent normal tissues were collected to examine the expressions of RhoE and EGFR by immunohistochemistry.The results and other relevant clinical parameters were used for statistical analysis.Results The average grey scale values of RhoE protein were(37.115 ±4.314)in ESCC tissues and(52.131 ±3.682)in adjacent normal tissues,and the difference was statistically significant(P <0.001).The average grey scale values of EGFR protein were(47.968 ±2.845)in ESCC tissues and(34.163 ±3.706)in adjacent normal tissues,the difference was also statistically significant(P <0.001).The expressions of RhoE and EGFR were associated with tumor infiltration,histological differentiation,lymph nodemetastasis and clinical stages(P <0.05),but they had no correlations with the size,location and generalclassifications of the tumors.The expressions of RhoE and EGFR in cancer tissue were negatively related(P <0.01).Conclusion EGFR expression is highly expressed in ESCC tissues while RhoE is antagonistic to it.These proteins are negatively correlated and they are associated with tumor infiltration,differentiation,lymph node metastasis and clinical stages.The results suggest that abnormal expression of EGFR andRhoE may participate in the occurrence and development of cancer,and testing RhoE and EGFR expression will be helpful to understand the pathogenesis and prognosis of ESCC.