CAJ | 학술논문

目的：观察乳腺浸润性导管癌组织中BRCA1、Ki-67及p53的表达及其与临床病理特征之间的关系。方法采用EliVisionTMplus两步免疫组化方法，检测BRCA1、Ki-67及P53在60例乳腺浸润性导管癌组织的表达情况。结果①60例乳腺浸润性导管癌组织中，BRCA1的阳性率为60．0％（36／60），Ki-67及 p53的阳性率分别为56．67％（34／60）、48．33％（29／60）；②BRCA1蛋白表达与患者发病年龄、临床分期、伴有腋淋巴结转移以及组织学分级有关（P＜0．05），而与肿瘤大小无关（P＞0．05）；Ki-67表达与临床分期、淋巴结转移、组织学分级有关（P＜0．05），与发病年龄、肿瘤大小无关（P＞0．05）；p53表达与肿瘤大小、临床分期、淋巴结转移、组织学分级相关（P＜0．05），与发病年龄无关（P＞0．05）；③BRCA1表达与 Ki-67、p53均呈负相关（r1＝－0．577，P＜0．05，r2＝－0．504，P ＜0．05），Ki-67与p53的表达呈正相关（r ＝0．375，P＜0．05）。结论联合检测BRCA1、Ki-67及p53对乳腺癌的早期诊断、恶性程度判定和预后监测有重要意义，可为乳腺癌的个体化治疗提供有效的指针。
목적：관찰유선침윤성도관암조직중BRCA1、Ki-67급p53적표체급기여림상병리특정지간적관계。방법채용EliVisionTMplus량보면역조화방법，검측BRCA1、Ki-67급P53재60례유선침윤성도관암조직적표체정황。결과①60례유선침윤성도관암조직중，BRCA1적양성솔위60．0％（36／60），Ki-67급 p53적양성솔분별위56．67％（34／60）、48．33％（29／60）；②BRCA1단백표체여환자발병년령、림상분기、반유액림파결전이이급조직학분급유관（P＜0．05），이여종류대소무관（P＞0．05）；Ki-67표체여림상분기、림파결전이、조직학분급유관（P＜0．05），여발병년령、종류대소무관（P＞0．05）；p53표체여종류대소、림상분기、림파결전이、조직학분급상관（P＜0．05），여발병년령무관（P＞0．05）；③BRCA1표체여 Ki-67、p53균정부상관（r1＝－0．577，P＜0．05，r2＝－0．504，P ＜0．05），Ki-67여p53적표체정정상관（r ＝0．375，P＜0．05）。결론연합검측BRCA1、Ki-67급p53대유선암적조기진단、악성정도판정화예후감측유중요의의，가위유선암적개체화치료제공유효적지침。
Objective ToinvestigatetheexpressionsofBRCA1,Ki-67andp53proteinininva-siveductalcarcinomaanddiscusstheircorrelationswithclinicopathologicalfeatures.Methods Theex-pressions of BRCA1,Ki-67and p53 were detected by immunohistochemistry with EliVisionTMplus two-step methodin60samplesofinvasiveductalcarcinomatissue.Results ThepositiverateofBRCA1protein was 60.0%(36/60).The positive rates of Ki-67 and p53 were 56.67%(34/60)and 48.33%(29/60). The expression of BRCA1 protein was related to the age of onset,clinical stage,lymph node metastasis and histological grade(P<0.05),but not associated with tumor size(P>0.05).The expression of Ki-67 was related to the clinical stage,lymph node metastasis and histological grade(P<0.05),but not associated with the age of onset or tumor size(P>0.05).The expression of p53 was related to the age of onset,clini-cal stage,lymph node metastasis and histological grade(P<0.05),but not associated with tumor size(P>0.05).The expression of BRCA1 protein in invasive ductal carcinoma was negatively correlated with Ki-67 and p53(r1 =-0.577,P<0.05,r2 =-0.504,P<0.05).The expression of Ki-67 was positively corre-latedwithp53(r=0.375,P<0.05).Conclusion CombineddetectionofBRCA1,Ki-67andp53isof important significance in early diagnosis,malignancy judgment and prognosis monitoring for invasive ductal carcinoma,which can provide effective indexes for individualized therapies.