CAJ | 학술논문

设计了一种新颖实用的制备L-别苏氨酸和D-别苏氨酸的方法。以α-C消旋的N-乙酰化苏氨酸为原料,用L-(或D-)氨基酰化酶拆分相应的底物得到了高光学纯度的L-别苏氨酸(或D-别苏氨酸)。为了获得较高收率,考查pH,温度,Co2+浓度对拆分反应的影响。确定最优条件为：pH 7．5,温度37℃,Co2+浓度0．1 g· L-1。反应产率达到93．8%( L-allo-Thr)、89．8%( D-allo-Thr),de>99%。该方法具有立体选择性高,操作简单,反应条件温和的优点。
설계료일충신영실용적제비L-별소안산화D-별소안산적방법。이α-C소선적N-을선화소안산위원료,용L-(혹D-)안기선화매탁분상응적저물득도료고광학순도적L-별소안산(혹D-별소안산)。위료획득교고수솔,고사pH,온도,Co2+농도대탁분반응적영향。학정최우조건위：pH 7．5,온도37℃,Co2+농도0．1 g· L-1。반응산솔체도93．8%( L-allo-Thr)、89．8%( D-allo-Thr),de>99%。해방법구유입체선택성고,조작간단,반응조건온화적우점。
A novel and practical approach for preparation of L-allothreonine and D-allothreonine was designed. The optical purity L-allo-Thr( or D-allo-Thr) compound was obtained by using the L-( or D-) aminoacylase to resolute the α-C racemic threonine of N-acetylation. The resolution conditions were optimized by assessing pH,temperature and the amount of Co2+ for obtaining the maxi-mum yield. The optimal condition was that pH was 7. 5,reaction temperature was 37℃,and concentration of Co2+was 0. 1 g·L-1 . The yield was 93. 8%for L-allo-Thr,89. 8%for D-allo-Thr,and de>99%. This method showed some advantages,such as high ste-reoselectivity,simple operation and mild conditions.