CAJ | 학술논문

目的：观察磷酸肌酸钠（ sodium creatine phosphate ，SCP）对离体大鼠肝脏中ALT／LDH指标变化，探讨磷酸肌酸在肝脏保护中的重要作用。方法选用Wister系雄性大鼠40只，普通级。应用大鼠肝脏单纯冷保存离体灌注模型，将40只大鼠随机分为对照组（单纯UW液灌注肝脏）、实验组以UW液为基液加入磷酸肌酸钠，依浓度分为高、中、低剂量组（浓度分别1 g／100 ml、2 g／100 ml、3 g／100 ml）。测定不同时间点保存液中丙氨酸氨基转移酶（ ALT）及乳酸脱氢酶（ LDH）活性。结果实验组在冷保存12 h后，ALT及LDH均低于对照组（ P ＜0¤．05），随着保存时间的延长，高、中、低剂量组的变化较对照组减轻，各剂量之间无差别（ P ＞0．05）。高剂量实验组在经过24 h冷保存后，肝组织保存液中ALT含量高于对照组及中、低剂量组（ P ＜0．05）。结论磷酸肌酸钠明显降低离体大鼠肝脏中ALT／LDH指标，对离体大鼠肝脏冷保存有较好的保护作用。
목적：관찰린산기산납（ sodium creatine phosphate ，SCP）대리체대서간장중ALT／LDH지표변화，탐토린산기산재간장보호중적중요작용。방법선용Wister계웅성대서40지，보통급。응용대서간장단순랭보존리체관주모형，장40지대서수궤분위대조조（단순UW액관주간장）、실험조이UW액위기액가입린산기산납，의농도분위고、중、저제량조（농도분별1 g／100 ml、2 g／100 ml、3 g／100 ml）。측정불동시간점보존액중병안산안기전이매（ ALT）급유산탈경매（ LDH）활성。결과실험조재랭보존12 h후，ALT급LDH균저우대조조（ P ＜0¤．05），수착보존시간적연장，고、중、저제량조적변화교대조조감경，각제량지간무차별（ P ＞0．05）。고제량실험조재경과24 h랭보존후，간조직보존액중ALT함량고우대조조급중、저제량조（ P ＜0．05）。결론린산기산납명현강저리체대서간장중ALT／LDH지표，대리체대서간장랭보존유교호적보호작용。
Objective To explore the protective effect of sodium phosphocreatine on rat livers ’ ALT/LDH of cold preservation , with the objective of providing an effective measure for clinical applications .Methods Simple cold preservation from rat liver perfusion model , Application of the experimental group with high , medium and low three different doses of sodium creatine phosphate UW solution and cryopreserved liver perfusion , the control group simplex UW liver perfusion and cryopreservation.Preservation solution was measured at different time points in alanine aminotransferase (ALT) and lactate dehydrogenase (LDH) activity.Result Different doses of the experimental group after 12h in cold storage,ALT and LDH were lower than the control group .After high dose experimental group after 24h cold preservation,ALT levels were higher in the low dose group.But the experiment found that high doses of the experimental group after group C after 24h cold preservation, ALT levels were higher and the A , B group,considering the drug concentration is too large ,increasing the length time cold preservation of the liver tissue destruction .Conclusions Sodium Phosphocreatine can decreas liver ’ ALT/LDH of cold preservation Significantly ,which has a good protective effect on isolated liver of cold preservation .