长江蔬菜
長江蔬菜
장강소채
JOURNAL OF CHANGJIANG VEGETABLES
2014年
24期
17-20
,共4页
藜蒿%茎段%快速繁殖%激素%浓度筛选
藜蒿%莖段%快速繁殖%激素%濃度篩選
려호%경단%쾌속번식%격소%농도사선
Artemisia selengensis%Stem%Rapid propagation%Plant growth hormone%Screening of concentration
以藜蒿茎段为外植体，通过对各组织培养阶段中激素种类和浓度的筛选，研究了不同激素使用的必要性及作用。试验结果表明，最适宜愈伤组织诱导的培养基分别为云南藜蒿 MS+6－BA 1.0 mg/L+IAA 0.5 mg/L、楚天藜蒿MS+6－BA 1.5 mg/L+IAA 1.0 mg/L、香藜一号 MS+6－BA 1.5 mg/L+IAA 0.5 mg/L；最适宜的增殖培养基分别为云南藜蒿 MS+6－BA 1.0 mg/L+NAA 0.1 mg/L，楚天藜蒿、香藜一号 MS+6－BA 1.0 mg/L+NAA 0.5 mg/L；最适宜的生根培养基为1/2 MS+NAA 0.1 mg/L。
以藜蒿莖段為外植體，通過對各組織培養階段中激素種類和濃度的篩選，研究瞭不同激素使用的必要性及作用。試驗結果錶明，最適宜愈傷組織誘導的培養基分彆為雲南藜蒿 MS+6－BA 1.0 mg/L+IAA 0.5 mg/L、楚天藜蒿MS+6－BA 1.5 mg/L+IAA 1.0 mg/L、香藜一號 MS+6－BA 1.5 mg/L+IAA 0.5 mg/L；最適宜的增殖培養基分彆為雲南藜蒿 MS+6－BA 1.0 mg/L+NAA 0.1 mg/L，楚天藜蒿、香藜一號 MS+6－BA 1.0 mg/L+NAA 0.5 mg/L；最適宜的生根培養基為1/2 MS+NAA 0.1 mg/L。
이려호경단위외식체，통과대각조직배양계단중격소충류화농도적사선，연구료불동격소사용적필요성급작용。시험결과표명，최괄의유상조직유도적배양기분별위운남려호 MS+6－BA 1.0 mg/L+IAA 0.5 mg/L、초천려호MS+6－BA 1.5 mg/L+IAA 1.0 mg/L、향려일호 MS+6－BA 1.5 mg/L+IAA 0.5 mg/L；최괄의적증식배양기분별위운남려호 MS+6－BA 1.0 mg/L+NAA 0.1 mg/L，초천려호、향려일호 MS+6－BA 1.0 mg/L+NAA 0.5 mg/L；최괄의적생근배양기위1/2 MS+NAA 0.1 mg/L。
Taking stem of Artemisia selengensis as explant, we studied the necessity and the effect of using different plant growth hormones to screen out suitable hormone types and concentrations in different culture stages. The results showed that, the most suitable medium for Yunnan A. selengensis callus induction was MS+6-BA 1.0 mg/L+IAA 0.5 mg/L, and the most suitable medium for Chutian A. selengensis callus induction was MS+6-BA 1.5 mg/L+IAA 1.0 mg/L, while the most suitable medium for Xiangli No.1 callus induction was MS +6 -BA 1.5 mg/L +IAA 0.5 mg/L. The most suitable enrichment medium for Yunnan A. selengensis was MS+6-BA 1.0 mg/L+NAA 0.1 mg/L, and the most enrichment suitable medium for Chutian A. selengensis and Xiangli No.1 was MS+6-BA 1.0 mg/L+NAA 0.5 mg/L, in addition, the most suitable medium for rooting of them was 1/2 MS+NAA 0.1 mg/L.
