CAJ | 학술논문

目的：探讨生物安全实验室采用气化过氧化氢（ H2 O2）消毒时H2 O2的最佳浓度及剂量。方法选择生物安全实验室一处，体积约120 m3，通过气化H2 O2消毒灭菌器（ VHP）将H2 O2溶液气化，采用9种不同的H2 O2注入速度及维持时间组合进行实验室消毒。消毒结束后，收集在实验室10个位置点放置的化学指示卡，观察化学指示卡颜色，收集各点放置的枯草杆菌黑色变种芽胞菌片，观察各点芽胞菌片平板的菌落生长情况。观察9种组合方法消毒后各实验点化学指示卡变化、生物指示菌片变化情况，选取消毒效果合格的下最少H2 O2剂量。按照最少剂量的参数设置再重复进行6次消毒实验，以验证消毒效果。结果 H2 O2使用剂量≥5 g／m3时，消毒实验后所有化学指示卡均由蓝色变为米黄色，消毒合格。选定最佳的H2 O2使用剂量为5 g／m3，6次消毒验证实验后，消毒效果全部合格。结论生物安全实验室采用气化H2 O2消毒时最佳浓度为35％，最佳剂量为5g／m3。
목적：탐토생물안전실험실채용기화과양화경（ H2 O2）소독시H2 O2적최가농도급제량。방법선택생물안전실험실일처，체적약120 m3，통과기화H2 O2소독멸균기（ VHP）장H2 O2용액기화，채용9충불동적H2 O2주입속도급유지시간조합진행실험실소독。소독결속후，수집재실험실10개위치점방치적화학지시잡，관찰화학지시잡안색，수집각점방치적고초간균흑색변충아포균편，관찰각점아포균편평판적균락생장정황。관찰9충조합방법소독후각실험점화학지시잡변화、생물지시균편변화정황，선취소독효과합격적하최소H2 O2제량。안조최소제량적삼수설치재중복진행6차소독실험，이험증소독효과。결과 H2 O2사용제량≥5 g／m3시，소독실험후소유화학지시잡균유람색변위미황색，소독합격。선정최가적H2 O2사용제량위5 g／m3，6차소독험증실험후，소독효과전부합격。결론생물안전실험실채용기화H2 O2소독시최가농도위35％，최가제량위5g／m3。
Objective To design the best dosage of vapor hydrogen peroxide in disinfection of high pathogenic micro -organism biosafety laboratory .Methods One room of 120 m3 in biosafety laboratory was selected .Hydrogen peroxide solu-tion was vaporized by hydrogen peroxide sterilizer ( VHP) .Nine types of vapor hydrogen peroxide inject speed and duration time were used to disinfect .After the disinfection , the chemical indicators and biological indicators were placed on 10 loca-tions in the room to observe the color of chemical indicators and the growth condition of all the plates inoculated with Bacil -lus subtilis var niger .After the 9 types of disinfection , the minimum vapor hydrogen peroxide was determined and then re-peated for 6 times in order to verify the disinfection effect .Results Qualified disinfection was proved that the color of the chemical indicators changed from orchid to ecru when the dosage no less than 5 g/M3 .The minimum vapor hydrogen perox-ide was determined at 5 g/m3 .After 6 times repeation of disinfection experiment at 5 g/m3 , all the disinfection results were qualified.Conclusions The best dosage of hydrogen peroxide solution with concentration of 35% was 5 g/m3 .It can reach the good efficacy in disinfection of air and surfaces in biosafety laboratory , which was suit for the disinfection in high pathogenic microorganism biosafety laboratory .