CAJ | 학술논문

目的：探讨体外环境下鼻咽癌高转移细胞株5-8F与淋巴管内皮细胞（ LEC）的相互影响。方法分别制备5-8F细胞及LEC的条件培养基（CM），即5-8FCM及LECCM。采用MTT法观察不同浓度5-8FCM对LEC增殖的影响；采用细胞划痕试验观察不同浓度LECCM对5-8F细胞运动迁移能力的影响。两项观察均设不同浓度RPMI-1640培养液为对照组。结果 MTT实验显示，培养24 h各浓度5-8FCM与相应的对照组比较，细胞增殖情况无统计学意义；培养48 h时10％、50％、75％及100％浓度的5-8FCM均能促进LEC增殖（P＜0．05）；培养72 h时75％与100％浓度的5-8FCM促进LEC增殖（ P＜0．05）。划痕试验表明，各浓度LECCM培养6、12、24 h时5-8F细胞迁移距离均大于相应的对照组（P均＜0．01）。结论体外培养时5-8FCM能促进LEC增殖；LECCM能显著促进5-8F细胞的迁移运动，二者具有交互作用，可共同促进鼻咽癌细胞的转移。
목적：탐토체외배경하비인암고전이세포주5-8F여림파관내피세포（ LEC）적상호영향。방법분별제비5-8F세포급LEC적조건배양기（CM），즉5-8FCM급LECCM。채용MTT법관찰불동농도5-8FCM대LEC증식적영향；채용세포화흔시험관찰불동농도LECCM대5-8F세포운동천이능력적영향。량항관찰균설불동농도RPMI-1640배양액위대조조。결과 MTT실험현시，배양24 h각농도5-8FCM여상응적대조조비교，세포증식정황무통계학의의；배양48 h시10％、50％、75％급100％농도적5-8FCM균능촉진LEC증식（P＜0．05）；배양72 h시75％여100％농도적5-8FCM촉진LEC증식（ P＜0．05）。화흔시험표명，각농도LECCM배양6、12、24 h시5-8F세포천이거리균대우상응적대조조（P균＜0．01）。결론체외배양시5-8FCM능촉진LEC증식；LECCM능현저촉진5-8F세포적천이운동，이자구유교호작용，가공동촉진비인암세포적전이。
Objective To investigate the interaction of highly metastatic cell line of nasopharyngeal carcinoma (5-8F) and lymphatic endothelial cell (LEC) in vitro.Methods The conditioned medium of 5-8F cell (5-8FCM) and LEC (LECCM) were prepared respectively .Methyl thiazolyl tetrazolium (MTT) assay was used to detect the effect of 5-8FCM in different concentrations on the proliferation of LEC .Scratch wound healing assay was performed to observe the effect of LECCM in different concentrations on migration of 5-8F cell.The RPMI-1640 medium in different concentrations were acted as the control group in both experiments .Results The result of MTT assay showed that no statistical difference of cellular proliferation was found when comparing the various 5-8FCM concentration groups with their corresponding control groups af-ter cultured 24 h.After cultured for 48 h, the proliferation of LEC was facilitated in 5-8FCM group with 10%, 50%, 75%and 100%concentration levels (P<0.05).After 72 h, the proliferation of LEC was promoted in 5-8FCM group with con-centration levels of 75%and 100%( P<0.05) .The result of scratch wound healing suggested that the migratory distance of 5-8F treated with LECCM of each concentration was completely longer than the corresponding control group (P<0.05) after cultured for 6, 12 and 24 h.Conclusions The 5-8FCM could promote the proliferation of LEC and the LECCM could significantly increase the migration of 5-8F in vitro.The interaction between 5-8F and LEC could collectively promote the metastasis of nasopharyngeal carcinoma .