CAJ | 학술논문

目的：建立八味芪丹胶囊中丹参素钠的含量测定方法。方法采用高效液相色谱法,色谱柱为Shim-pack CLC-ODS C18(250 mm×4.6 mm,5μm),流动相为甲醇-水-冰醋酸溶液(7∶92∶1,V/V/V),检测波长为281 nm,柱温30℃,流速1.0 mL/min,进样量10μL。结果通过方法学考察,丹参素钠线性范围为0.4944～4.944μg(r＝0.9996),平均加样回收率为97.20%(RSD＝1.13%)。结论所建立的八味芪丹胶囊中丹参素钠的HPLC检测方法操作简便,准确可靠,具有较好的重复性与稳定性,适用于八味芪丹胶囊的质量控制。
목적：건립팔미기단효낭중단삼소납적함량측정방법。방법채용고효액상색보법,색보주위Shim-pack CLC-ODS C18(250 mm×4.6 mm,5μm),류동상위갑순-수-빙작산용액(7∶92∶1,V/V/V),검측파장위281 nm,주온30℃,류속1.0 mL/min,진양량10μL。결과통과방법학고찰,단삼소납선성범위위0.4944～4.944μg(r＝0.9996),평균가양회수솔위97.20%(RSD＝1.13%)。결론소건립적팔미기단효낭중단삼소납적HPLC검측방법조작간편,준학가고,구유교호적중복성여은정성,괄용우팔미기단효낭적질량공제。
Objective To establish an HPLC method for the content determination of sodium Danshensu inBawei Qidan Capsule.Methods HPLC was applied and the determination was performed on Shim-pack CLC-ODS C18 column (250 mm×4.6 mm, 5μm) with methanol-water- acetic acid (7∶92∶1, V/V/V) solution at a flow rate of 1.0 mL/min and detection wavelength at 281 nm. The column temperature was 30℃, and injection volume was 10μL.Results Through methodological study, the linear range of sodium Danshensu was 0.494 4-4.944μg (r=0.9996), the average recovery was 97.20%, RSD=1.13%.Conclusion The HPLC method for the content determination of sodium Danshensu inBawei QidanCapsule was user-friendly, accurate and reliable, with good repeatability and stability, which can be used for quality control ofBawei Qidan Capsule.