CAJ | 학술논문

目的：建立清咽颗粒的质量标准。方法采用薄层色谱法对清咽颗粒中的主要药味山豆根、南沙参、忍冬藤、麦冬进行定性鉴别。采用高效液相色谱法测定甘草中甘草苷、甘草酸的含量,色谱柱为Thermo Syncronis C18(250 mm×4.6 mm,5μm),流动相为乙腈(含0.05%磷酸,A)-0.05%磷酸溶液(B),梯度洗脱(0～8 min,19%A；8～35 min,19%→50%A),检测波长237 nm,流速1 mL/min。结果薄层色谱斑点清晰,在与对照品或对照药材相应的位置上显相同颜色的斑点,阴性对照无干扰。甘草苷的线性范围为0.05～0.5μg,r＝0.9999,平均回收率为99.97%,RSD＝1.74%(n＝9)；甘草酸铵的线性范围为0.1～2μg,r＝0.9999,平均回收率为99.74%,RSD＝1.28%(n＝9)。结论本方法简便可行,结果准确可靠,重复性好,可作为清咽颗粒的质量控制方法。
목적：건립청인과립적질량표준。방법채용박층색보법대청인과립중적주요약미산두근、남사삼、인동등、맥동진행정성감별。채용고효액상색보법측정감초중감초감、감초산적함량,색보주위Thermo Syncronis C18(250 mm×4.6 mm,5μm),류동상위을정(함0.05%린산,A)-0.05%린산용액(B),제도세탈(0～8 min,19%A；8～35 min,19%→50%A),검측파장237 nm,류속1 mL/min。결과박층색보반점청석,재여대조품혹대조약재상응적위치상현상동안색적반점,음성대조무간우。감초감적선성범위위0.05～0.5μg,r＝0.9999,평균회수솔위99.97%,RSD＝1.74%(n＝9)；감초산안적선성범위위0.1～2μg,r＝0.9999,평균회수솔위99.74%,RSD＝1.28%(n＝9)。결론본방법간편가행,결과준학가고,중복성호,가작위청인과립적질량공제방법。
Objective To establish the standard for quality control ofQingyan Granule. Methods The chief components of the preparation, Sophora Tonkinensis radix et rhizoma, Adenophorae radix, Lonicera japonica caulis, and Ophiopogonis radix were identified by TLC qualitatively. The contents of licorice glycosides and glycyrrhizic acid were determined by HPLC. The separation was performed on Thermo Syncronis C18 column (4.6 mm×250 mm, 5μm) with mobile phase consisted of acetonitrile with 0.05% phosphoric acid solution (A)-0.05% phosphoric acid solution (B), and gradient elution (0-8 min, 19%A;8-35 min, 19%→50%A). Detection wavelength was 237 nm, and flow rate was 1 mL/min.Results The spots in TLC were clear. There were spots with same color on the corresponding location of reference substance and reference herbal, negative control without interference. The linear range for licorice glycosides was 0.05-0.5μg (r=0.999 9). The average recovery was 99.97%, RSD=1.74% (n=9). The linear range for glycyrrhizic acid was 0.1-2μg (r=0.999 9). The average recovery was 99.74%, RSD=1.28% (n=9). Conclusion The method is simple, accurate, with high reproducibility, which can be used for quality control ofQingyan Granule.