CAJ | 학술논문

目的：提高祖师麻膏药的质量标准。方法采用薄层色谱法对祖师麻膏药中祖师麻甲素和7-羟基香豆素进行定性鉴别。采用高效液相色谱法对祖师麻膏药中祖师麻甲素和7-羟基香豆素进行含量测定,色谱柱为Agilent Eclipse Plus C18柱(4.6 mm×250 mm,5μm),流动相为乙腈-0.1%磷酸水溶液(15∶85,V/V),流速为1 mL/min,检测波长为327 nm,柱温为30℃。结果样品中检出祖师麻甲素和7-羟基香豆素的特征斑点,图谱斑点清晰、专属性强,阴性对照无干扰。祖师麻甲素和7-羟基香豆素分别在0.180～1.800μg和0.100～1.00μg范围内与其峰面积呈良好线性关系(r＝0.9991,r＝0.9992),平均加样回收率分别为98.99%、101.48%,RSD分别为1.43%、1.32%(n＝9)。结论所建立的提高标准方法简便准确,专属性强,可用于该制剂的质量控制。
목적：제고조사마고약적질량표준。방법채용박층색보법대조사마고약중조사마갑소화7-간기향두소진행정성감별。채용고효액상색보법대조사마고약중조사마갑소화7-간기향두소진행함량측정,색보주위Agilent Eclipse Plus C18주(4.6 mm×250 mm,5μm),류동상위을정-0.1%린산수용액(15∶85,V/V),류속위1 mL/min,검측파장위327 nm,주온위30℃。결과양품중검출조사마갑소화7-간기향두소적특정반점,도보반점청석、전속성강,음성대조무간우。조사마갑소화7-간기향두소분별재0.180～1.800μg화0.100～1.00μg범위내여기봉면적정량호선성관계(r＝0.9991,r＝0.9992),평균가양회수솔분별위98.99%、101.48%,RSD분별위1.43%、1.32%(n＝9)。결론소건립적제고표준방법간편준학,전속성강,가용우해제제적질량공제。
Objective To improve the quality standard ofZushima Plaster.Methods TLC was used to do qualitative identification of daphnetin and 7-hydroxycoumarin inZushima Plaster. The contents of daphnetin and 7-hydroxycoumarin inZushima Plaster were determined by HPLC. The chromatographic column was Agilent Eclipse Plus C18 (4.6 mm×250 mm, 5μm), and the mobile phase consisted of acetonitrile-0.1% phosphoric asid (15∶85, V/V) at a flow rate of 1.0 mL/min. The column temperature was maintained at 30℃, and the detection wavelength was set at 327 nm. Results Characteristic spots of daphnetin and 7-hydroxycoumarin could be detected by TLC from the samples, and the spots were clear and specific without interference from negative samples. The calibration curve of daphnetin and 7-hydroxycoumarin were linear in the ranges of 0.180-1.800μg and 0.100-1.00μg (r=0.999 1,r=0.999 2), and the average recovery rates were 98.99% and 101.48%, RSDwere1.43% and 1.32%, respectively (n=9).Conclusion The established method appears easy to use, accurate and specific, and therefore can be used for the quality control ofZushima Plaster.