CAJ | 학술논문

目的：建立紫朱软膏中紫草的质量控制方法。方法采用薄层色谱法对紫朱软膏中紫草进行定性鉴别。以左旋紫草素含量为评价指标,采用正交试验设计优选左旋紫草素的提取工艺,以紫外可见分光光度法对左旋紫草素进行含量测定。结果在薄层色谱中,主斑点清晰,阴性对照无干扰。左旋紫草素的最佳提取工艺为：提取3次,每次30 min,NaOH体积分数为1%、用量为50 mL。左旋紫草素在0.0102～0.0357 mg(r2＝0.9997)范围内与吸收度呈良好的线性关系,平均加样回收率为103.27%,RSD＝4.17%。结论本方法简便可行,可用于紫朱软膏的质量控制和评价。
목적：건립자주연고중자초적질량공제방법。방법채용박층색보법대자주연고중자초진행정성감별。이좌선자초소함량위평개지표,채용정교시험설계우선좌선자초소적제취공예,이자외가견분광광도법대좌선자초소진행함량측정。결과재박층색보중,주반점청석,음성대조무간우。좌선자초소적최가제취공예위：제취3차,매차30 min,NaOH체적분수위1%、용량위50 mL。좌선자초소재0.0102～0.0357 mg(r2＝0.9997)범위내여흡수도정량호적선성관계,평균가양회수솔위103.27%,RSD＝4.17%。결론본방법간편가행,가용우자주연고적질량공제화평개。
Objective To establish a method for quality control of Arnebia euchroma inZizhu Ointment.Methods The TLC was used to make qualitative identification to Arnebia euchroma in Zizhu Ointment. The content of shikonin was targeted as the evaluation index and the method of orthogonal design was used to optimize the extraction of shikonin. UV-visible spectrophotometry was used for the determination of shikonin.Results Main spots of TLC were clear, and negative control had no interference. The optimum extraction of shikonin was extracted for three times, 30 min for each time, NaOH volume fraction of 1%, NaOH amount of 50 mL. The absorbance of shikonin showed a good linear relationship in the range of 0.010 2-0.035 7 mg (r2=0.999 7), and the average recovery was 103.27%, RSD=4.17%.Conclusion This method was simple and feasible, and can be used for the quality control and the evaluation ofZizhu Ointment.