CAJ | 학술논문

目的：探讨四妙散药物血清防治肾间质纤维化的作用机制。方法将人肾小管上皮细胞(HK-2)分为空白组、模型组、对照组及药物血清高、中、低剂量组。实时荧光定量PCR检测结缔组织生长因子(CTGF)、骨形成蛋白-7(BMP-7),免疫组织化学检测CTGF、BMP-7蛋白的表达。结果尿酸诱导后CTGF的mRNA和蛋白表达量明显增加(P＜0.05),BMP-7的mRNA和蛋白表达量明显降低(P＜0.05)；经药物血清干预后,随剂量增加CTGF的表达逐步下降(P＜0.05),BMP-7的表达逐步上升(P＜0.05),呈现一定剂量依赖性。结论四妙散抗肾间质纤维化的机制可能是通过下调CTGF的表达和上调BMP-7的表达抑制上皮细胞转分化完成的。
목적：탐토사묘산약물혈청방치신간질섬유화적작용궤제。방법장인신소관상피세포(HK-2)분위공백조、모형조、대조조급약물혈청고、중、저제량조。실시형광정량PCR검측결체조직생장인자(CTGF)、골형성단백-7(BMP-7),면역조직화학검측CTGF、BMP-7단백적표체。결과뇨산유도후CTGF적mRNA화단백표체량명현증가(P＜0.05),BMP-7적mRNA화단백표체량명현강저(P＜0.05)；경약물혈청간예후,수제량증가CTGF적표체축보하강(P＜0.05),BMP-7적표체축보상승(P＜0.05),정현일정제량의뢰성。결론사묘산항신간질섬유화적궤제가능시통과하조CTGF적표체화상조BMP-7적표체억제상피세포전분화완성적。
Objective;To explore the mechanism ofSimiaosan drug-containing serum on prevention and treatment of renal fibrosis.Methods Human renal tubular epithelial cells (HK-2) were divided into blank group, model group, control group, and drug-containing serum high dosage group, medium dosage group and low dosage group. The expressions of CTGF and BMP-7 were determined by real-time PCR assay and immunocytochemistry.Results After the HK-2 cell was induced by uric acid (UA), the expression of CTGF significantly increased (P<0.05), the expression of BMP-7 significantly decreased (P<0.05). After intervened bySimiaosan, the expression of CTGF significantly decreased (P<0.05), the expression of BMP-7 significantly increased (P<0.05) and present dosage dependent.Conclusion Reducing the expression of CTGF and up-regulation the expression of BMP-7 and controlling epithelial-myofibroblast transdifferentiation may be the mechanism ofSimiaosan on prevention and treatment of renal fibrosis.