CAJ | 학술논문

目的：探讨调中化痰安神合剂镇静催眠的作用机制。方法健康昆明种小鼠200只,随机分为4组各50只,各组又随机分为空白组、对照组和调中化痰安神合剂高、中、低剂量组(中药高、中、低剂量组),每组10只,分别予生理盐水、艾司唑仑和高、中、低剂量调中化痰安神合剂灌胃给药,每日1次。第1组连续给药7 d,观察各组小鼠给药前后自发活动的次数。第2组连续给药7 d,给药结束60 min后,各组小鼠予戊巴比妥钠30 mg/kg体质量(引起100%小鼠睡眠的最小阈剂量)腹腔注射,记录各组小鼠的睡眠时间。第3组连续给药7 d,给药结束60 min后,各组小鼠予戊巴比妥钠15 mg/kg体质量(引起90%～100%小鼠翻正反射不消失的最大剂量)腹腔注射,观察并记录入睡动物数,计算入睡率。第4组连续给药15 d后脱颈处死,迅速取出脑组织,分批取出匀浆,荧光分光光度法测定小鼠脑组织中5-羟色胺(5-HT)、5-羟吲哚乙酸(5-HIAA)含量。结果与空白组比较,各给药组小鼠活动次数减少、睡眠潜伏期缩短、睡眠持续时间延长、入睡率提高(P＜0.01,P＜0.05),其中对照组和中药高剂量组镇静效果明显优于其他给药组,差异有统计学意义(P＜0.05),但2组比较差异未见统计学意义(P＞0.05)。与空白组比较,各给药组小鼠脑内5-HT、5-HIAA 含量增加,差异有统计学意义(P＜0.01,P＜0.05),中药高剂量组增加最显著(P＜0.05)。结论调中化痰安神合剂有明显镇静催眠作用,其作用机制可能与增加脑内5-HT合成与代谢有关。
목적：탐토조중화담안신합제진정최면적작용궤제。방법건강곤명충소서200지,수궤분위4조각50지,각조우수궤분위공백조、대조조화조중화담안신합제고、중、저제량조(중약고、중、저제량조),매조10지,분별여생리염수、애사서륜화고、중、저제량조중화담안신합제관위급약,매일1차。제1조련속급약7 d,관찰각조소서급약전후자발활동적차수。제2조련속급약7 d,급약결속60 min후,각조소서여무파비타납30 mg/kg체질량(인기100%소서수면적최소역제량)복강주사,기록각조소서적수면시간。제3조련속급약7 d,급약결속60 min후,각조소서여무파비타납15 mg/kg체질량(인기90%～100%소서번정반사불소실적최대제량)복강주사,관찰병기록입수동물수,계산입수솔。제4조련속급약15 d후탈경처사,신속취출뇌조직,분비취출균장,형광분광광도법측정소서뇌조직중5-간색알(5-HT)、5-간신타을산(5-HIAA)함량。결과여공백조비교,각급약조소서활동차수감소、수면잠복기축단、수면지속시간연장、입수솔제고(P＜0.01,P＜0.05),기중대조조화중약고제량조진정효과명현우우기타급약조,차이유통계학의의(P＜0.05),단2조비교차이미견통계학의의(P＞0.05)。여공백조비교,각급약조소서뇌내5-HT、5-HIAA 함량증가,차이유통계학의의(P＜0.01,P＜0.05),중약고제량조증가최현저(P＜0.05)。결론조중화담안신합제유명현진정최면작용,기작용궤제가능여증가뇌내5-HT합성여대사유관。
Objective To explore the mechanism ofTiaozhong Huatan Anshen Mixture (THAM). Methods A total of 200 healthy Kunming mice were randomly and equally divided into first, second, third and fourth major groups. Each major group was further equally divided into blank group, control group and high, medium, low dose THAM groups. Each group was given an equal volume of NS, estazolam, or high, medium, or low dose THAM by gavage for 7 d. For the first major group, the changes in number of spontaneous activities after administration were evaluated. For the second major group, 60 min after administration, each group was given pentobarbital sodium (30 mg/kg body weight, minimum dose for all mice sleeping) by intraperitoneal injection, and the sleep time was recorded. For the third major group, 60 min after administration, each group was given pentobarbital sodium (15 mg/kg body weight, maximum dose for 90%-100% of all mice having righting reflex) by intraperitoneal injection. The number of mice falling asleep was recorded, and the sleep rate was calculated. For the fourth major group, mice were sacrificed after continuous administration 15 d. They were quickly removed brain tissue;homogenates were taken out in batches;5-HT and 5-HIAA levels in brain tissues were determined according to fluorescence spectrophotometric.Results Compared with the blank group, all treated groups had a reduced number of spontaneous activities, a shortened sleep latency, a prolonged sleep time, and an increased sleep rate after administration (P<0.01,P<0.05), with the most significant changes seen in the control group and high-dose THAM group (P<0.05), with out statistical significance between the two groups (P>0.05). Compared with the blank group, brain 5-HT and 5-HIAA levels in all treated groups increased in mice (P<0.01,P<0.05), with the most significant changes seen in the high-dose THAM group (P<0.05).Conclusion THAM has a significant sedative-hypnotic effect. The mechanism may be related to increasing brain 5-HT synthesis and metabolism.