CAJ | 학술논문

目的：探讨血通灵对人乳腺癌细胞MDA-MB-231侵袭转移作用机制。方法 Transwell侵袭实验检测不同浓度血通灵对细胞侵袭作用的影响，初步明确药物抗肿瘤转移的物质基础；应用明胶酶谱法检测血通灵对MDA-MB-231细胞分泌基质金属蛋白酶-9(MMP-9)的影响；Western blot检测不同浓度血通灵干预后细胞中MMP-9蛋白表达的变化。结果不同浓度血通灵均能显著抑制细胞分泌 MMP-9能力、细胞侵袭能力和细胞内MMP-9蛋白表达，该抑制作用呈剂量依赖关系。结论血通灵可能通过下调MDA-MB-231细胞中MMP-9蛋白表达而降低活性MMP-9的分泌，抑制细胞的侵袭能力。
목적：탐토혈통령대인유선암세포MDA-MB-231침습전이작용궤제。방법 Transwell침습실험검측불동농도혈통령대세포침습작용적영향，초보명학약물항종류전이적물질기출；응용명효매보법검측혈통령대MDA-MB-231세포분비기질금속단백매-9(MMP-9)적영향；Western blot검측불동농도혈통령간예후세포중MMP-9단백표체적변화。결과불동농도혈통령균능현저억제세포분비 MMP-9능력、세포침습능력화세포내MMP-9단백표체，해억제작용정제량의뢰관계。결론혈통령가능통과하조MDA-MB-231세포중MMP-9단백표체이강저활성MMP-9적분비，억제세포적침습능력。
Objective To research the action mechanism of Chinese FormulaXuetongling on MDA-MB-231 human breast cancer cell invasion.Methods Transwell Invasion assay was applied to investigate the inhibitory effects on cancer cell invasion ofXuetongling extract in different concentrations;MMP-9 secretion activity was detected by zymography assay after the treatment of XTL extract in MDA-MB-231;Western blot was used to detect the effect of XTL extract on MMP-9 protein expression in MDA-MB-231.ResultsXuetongling extract in different concentrations significantly suppressed the cell invasion, MMP-9 secretion and MMP-9 protein expression in dose dependent manner.Conclusion The inhibitory effect ofXuetongling on MDA-MB-231 cell invasion may be due to the down-regulation of both MMP-9 secretion and MMP-9 protein expression.