CAJ | 학술논문

目的：探讨红芪黄酮防治肺纤维化的作用机制。方法 SPF级Wistar大鼠216只,分为正常组、模型组、强的松组及红芪黄酮高、中、低剂量组。采用气管内滴注博莱霉素法建立肺纤维化模型,从造模后第2日起,各药物组分别给予相应剂量药物,按时间点连续灌胃治疗7、14、28 d,免疫组化法检测基质金属蛋白酶(MMP)-2、基质金属蛋白酶抑制剂(TIMP)-1蛋白的表达。结果红芪黄酮高剂量组第7、14、28日及红芪黄酮中剂量组第14日MMP-2蛋白的表达较模型组明显减轻,差异有统计学意义(P＜0.05),红芪黄酮高剂量组表达水平与强的松组相似；红芪黄酮高、中剂量组第14日及红芪黄酮高剂量组第28日TIMP-1蛋白表达均较模型组明显减轻,差异有统计学意义(P＜0.05)。结论红芪黄酮在各个时点调整MMP-2和TIMP-1的蛋白表达,使MMPs/TIMPs趋于平衡,可能是其抑制肺纤维化进程的一种有效机制。
목적：탐토홍기황동방치폐섬유화적작용궤제。방법 SPF급Wistar대서216지,분위정상조、모형조、강적송조급홍기황동고、중、저제량조。채용기관내적주박래매소법건립폐섬유화모형,종조모후제2일기,각약물조분별급여상응제량약물,안시간점련속관위치료7、14、28 d,면역조화법검측기질금속단백매(MMP)-2、기질금속단백매억제제(TIMP)-1단백적표체。결과홍기황동고제량조제7、14、28일급홍기황동중제량조제14일MMP-2단백적표체교모형조명현감경,차이유통계학의의(P＜0.05),홍기황동고제량조표체수평여강적송조상사；홍기황동고、중제량조제14일급홍기황동고제량조제28일TIMP-1단백표체균교모형조명현감경,차이유통계학의의(P＜0.05)。결론홍기황동재각개시점조정MMP-2화TIMP-1적단백표체,사MMPs/TIMPs추우평형,가능시기억제폐섬유화진정적일충유효궤제。
Objective To discuss radix hedysari flavonoids mechanism of preventing pulmonary fibrosis.Methods Totally 216 SPF Wistar rats were randomized into normal group, model group, prednisone group, radix hedysari flavonoids high, medium, and low dose groups, and then pulmonary fibrosis model was established by intratracheal dripping of bleomycin. From the second day after modeling, every treatment group received gavage with the corresponding dose of medicine at the planned time for 7, 14, and 28 continuous days. Expressions of MMP-2 and TIMP-1 protein were detected by immunohistochemical method.Results When radix hedysari flavonoids high dose group was at the 7th, 14th, 28th day, and medium dose group was at the 14th day, MMP-2 protein expression was lower than model group, similar to prednisone group. When radix hedysari flavonoids high dose group was at the 14th, 28th day and medium dose group was at 14th day, TIMP-1 protein expression was lower than model group.Conclusion Radix hedysari flavonoids can adjust the expressions of MMP-2 and TIMP-1 protein at different phases, and tend to make the balance of MMPs/TIMPs, which may be an effective mechanism for the inhibition of fibrosis process.