CAJ | 학술논문

目的：探讨TOPA2基因在三阴性乳腺癌中的表达特点及其与中医体质类型的相关性。方法对符合纳入标准的71例三阴性乳腺癌患者进行体质辨识，统计各体质类型分布情况。采用实时荧光定量聚合酶链反应对有肿瘤组织残存的组织蜡块进行TOP2A基因检测。采用相对定量2-ΔΔCt法分析各个样本目的基因表达的变化。采用单因素相关法对体质类型分布情况(频数)与TOP2A基因扩增表达(表达率)进行相关性分析，采用SPSS17.0统计软件进行分析。结果71例患者可分为平和质(0例)、气虚质(18例)、阳虚质(10例)、阴虚质(12例)、气郁质(7例)、血瘀质(10例)、痰湿质(8例)、湿热质(6例)、特禀质(0例)，均为偏颇体质，气虚质所占比例最大(25.35%)，阴虚质(16.90%)和血瘀质(14.08%)次之。TOP2A 基因扩增表达与各中医体质呈负相关(P＜0.05)。结论三阴性乳腺癌人群体质主要为偏颇体质，以气虚质、阴虚质和血瘀质为主。三阴性乳腺癌常见发病体质中的TOP2A基因表达呈正常或缺失状态，提示化疗敏感性低，预后差。
목적：탐토TOPA2기인재삼음성유선암중적표체특점급기여중의체질류형적상관성。방법대부합납입표준적71례삼음성유선암환자진행체질변식，통계각체질류형분포정황。채용실시형광정량취합매련반응대유종류조직잔존적조직사괴진행TOP2A기인검측。채용상대정량2-ΔΔCt법분석각개양본목적기인표체적변화。채용단인소상관법대체질류형분포정황(빈수)여TOP2A기인확증표체(표체솔)진행상관성분석，채용SPSS17.0통계연건진행분석。결과71례환자가분위평화질(0례)、기허질(18례)、양허질(10례)、음허질(12례)、기욱질(7례)、혈어질(10례)、담습질(8례)、습열질(6례)、특품질(0례)，균위편파체질，기허질소점비례최대(25.35%)，음허질(16.90%)화혈어질(14.08%)차지。TOP2A 기인확증표체여각중의체질정부상관(P＜0.05)。결론삼음성유선암인군체질주요위편파체질，이기허질、음허질화혈어질위주。삼음성유선암상견발병체질중적TOP2A기인표체정정상혹결실상태，제시화료민감성저，예후차。
Objective To explore the correlation between TOP2A gene expression and TCM constitutional types of patients with triple-negative breast cancer.Methods The questionnaire for constitution of Chinese medicine was used to identify the TCM constitutional types of 71 patients with triple-negative breast cancer and TOP2A gene alterations were quantified by real-time PCR. 2-ΔΔCt was used to analyze the expressions of each group. SPSS17.0 was employed to analyze the correlation between TOP2A gene amplification and TCM constitutional types.Results Totally 71 patients were divided into general type (0 case), Qi deficiency type (18 cases), Yang deficiency type (10 cases), Yin deficiency type (12 cases), Qi stagnancy type (7 cases), blood stasis type (10 cases), phlegm wet calculus type (8 cases), damp-heat type (6 cases), and special type (0 case). All of them were biased constitutional types, of which Qi deficiency constitution (25.35%), Yin deficiency constitution (16.90%), blood stasis constitution (14.08%) took the majority. TOP2A gene amplification was negative correlated with each constitutional type (P<0.05).Conclusion Biased constitutional types were the main types of TCM constitutional type of patients with triple-negative breast cancer. The main three biased constitutional types of triple-negative breast cancer were Qi deficiency constitution, Yin deficiency constitution and blood stasis constitution. TOP2A gene expression in patients with triple-negative breast cancer was normal or less expressed, which predicted low chemotherapy sensitivity and poor prognosis.