CAJ | 학술논문

采用正己烷提取,薄层色谱纯化,高效液相色谱法(HPLC)检测并考察几种银杏类制剂总银杏酸的含量。色谱条件为Hypersil ODS C-18(150 mm×4.6 mm,5μm)；流动相组成为甲醇-3% HAc溶液(92：8, V/V),紫外检测波长306 nm,流速1.0 ml/ min,柱温40℃,进样量：20μl,外标法计算含量。结果表明：总银杏酸线性回归方程为：Y=4998X-100492,r2=0.9986,线性关系良好。标准品和样品的相对标准偏差RSD均为0.38%,表明仪器精密度良好。7种银杏产品中,采摘的新鲜银杏叶中总银杏酸的含量接近于1%,其余6种市场上的银杏制剂总银杏酸含量均较大程度超过10 ppm。其中,这一研究结果为正确使用银杏叶产品提供了参考。
채용정기완제취,박층색보순화,고효액상색보법(HPLC)검측병고찰궤충은행류제제총은행산적함량。색보조건위Hypersil ODS C-18(150 mm×4.6 mm,5μm)；류동상조성위갑순-3% HAc용액(92：8, V/V),자외검측파장306 nm,류속1.0 ml/ min,주온40℃,진양량：20μl,외표법계산함량。결과표명：총은행산선성회귀방정위：Y=4998X-100492,r2=0.9986,선성관계량호。표준품화양품적상대표준편차RSD균위0.38%,표명의기정밀도량호。7충은행산품중,채적적신선은행협중총은행산적함량접근우1%,기여6충시장상적은행제제총은행산함량균교대정도초과10 ppm。기중,저일연구결과위정학사용은행협산품제공료삼고。
A detection method of ginkgolic acids in ginkgo preparations by high performance liquid chromatography (HPLC) was established with extraction of n-hexane and purification of thin-layer chromatography. The chromatographic condition was Hypersil ODS C-18 (150 mm×4.6 mm, 5μm). The composition of mobile phase was methanol-3% HAc solution (92:8, V/V). Ultraviolet detection wavelength was 306 nm, flow velocity was 1.0 ml/ min, column temperature was 40℃, and sample amount was 20μl. The content was calculated by external standard method. The results showed that the linear regression equation of ginkgolic acids wasY=4998X-100492,r2=0.9986, and the linear relation was good. The relative standard deviations RSD of standard and extraction samples were all 0.38%, which showed the instrument had good precision. In 7 ginkgo samples, the content of ginkgolic acids in fresh ginkgo leaf was 1%, and that of the other 6 ginkgo drugs were all above 10 ppm. This study provided references for correct use of ginkgo preparations.