CAJ | 학술논문

目的：探讨ALOX5AP SG13S114A／T、COX-2765G／C和COX-150C／T多态性及交互作用与脑梗死的相关性。方法：采用聚合酶链反应－单核苷酸多态性方法对411例脑梗死患者（脑梗死组）和411名对照者（对照组）ALOX5AP基因SG13S114A／T、COX-2基因765G／C和COX-1基因50C／T位点多态性进行检测，应用广义多因子降维法（GMDR）检测基因与基因的交互作用。结果：ALOX5AP SG13S114 A／T、COX-150C／T和COX-2765G／C基因型及等位基因分布在脑梗死组与对照组比较差异均无统计学意义；但GMDR示SG13S114和COX-2具有联合交互作用，同时携带ALOX5AP SG13S114AA型和COX-2765CC基因型者脑梗死风险增加2．842倍。结论：对基因与基因的交互作用进行分析有助于更深入研究复杂疾病的基因型和表型间的关系。
목적：탐토ALOX5AP SG13S114A／T、COX-2765G／C화COX-150C／T다태성급교호작용여뇌경사적상관성。방법：채용취합매련반응－단핵감산다태성방법대411례뇌경사환자（뇌경사조）화411명대조자（대조조）ALOX5AP기인SG13S114A／T、COX-2기인765G／C화COX-1기인50C／T위점다태성진행검측，응용엄의다인자강유법（GMDR）검측기인여기인적교호작용。결과：ALOX5AP SG13S114 A／T、COX-150C／T화COX-2765G／C기인형급등위기인분포재뇌경사조여대조조비교차이균무통계학의의；단GMDR시SG13S114화COX-2구유연합교호작용，동시휴대ALOX5AP SG13S114AA형화COX-2765CC기인형자뇌경사풍험증가2．842배。결론：대기인여기인적교호작용진행분석유조우경심입연구복잡질병적기인형화표형간적관계。
Objective To investigate the interrelations of ALOX5AP SG13S114A/T , COX-2 765G/C , COX-1-50C/T polymorphisms and cerebral infarction. Methods The ALOX5AP SG13S114A/T, COX-2 765G/C and COX-1 50C/T polymorphisms in 411 cases with cerebral infarction and 411 controls were measured by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism method. The generalized multifactor dimensionality reduction (GMDR) method was employed to detect gene-gene interactions. Results Single-gene analysis showed that there were no significant differences in the genotype and allele frequency distributions of ALOX5AP SG13S114A/T, COX-2 765G/C and COX-1 50C/T between two groups. However, in those cases carrying ALOX5AP SG13S114AA as well as COX-2 765CC , the risk of cerebral infarction increased significantly by 2.842 times. Conclusions The combinational analysis among genes used in this study may be helpful in the elucidation of genetic risk factors for common and complex diseases.